TY - JOUR
T1 - Degradation of carbamazepine by Trametes versicolor in an air pulsed fluidized bed bioreactor and identification of intermediates
AU - Jelic, Aleksandra
AU - Cruz-Morató, Carles
AU - Marco-Urrea, Ernest
AU - Sarrà, Monserrat
AU - Perez, Sandra
AU - Vicent, Teresa
AU - Petrović, Mira
AU - Barcelo, Damia
PY - 2012/3/15
Y1 - 2012/3/15
N2 - The paper describes the aerobic degradation of carbamazepine (CBZ), an anti-epileptic drug widely found in aquatic environment, from Erlenmeyer flask to bioreactor by the white-rot fungus Trametes versicolor. In Erlenmeyer flask, CBZ at approximately 9mgL-1 was almost completely eliminated (94%) after 6d, while at near environmentally relevant concentrations of 50μgL-1, 61% of the contaminant was degraded in 7d. Acridone, acridine, 10,11-dihydro-10,11-dihydroxy-CBZ, and 10, 11-epoxy-CBZ were identified as major metabolites, confirming the degradation of CBZ. The degradation process was then carried out in an air pulsed fluidized bioreactor operated in batch and continuous mode. Around 96% of CBZ was removed after 2 days in batch mode operation, and 10,11-dihydro-10,11-epoxycarbamazepine was found as unique metabolite. In bioreactor operated in continuous mode with a hydraulic retention time of 3d, 54% of the inflow concentration (approx. 200μgL-1) was reduced at the steady state (25d) with a CBZ degradation rate of 11.9μgCBZg-1 dry weight d-1. No metabolite was detected in the culture broth. Acute toxicity tests (Microtox) indicated that the final culture broth in both batch and continuous mode operation were non toxic, with 15min EC50 values of 24% and 77%, respectively. © 2011 Elsevier Ltd.
AB - The paper describes the aerobic degradation of carbamazepine (CBZ), an anti-epileptic drug widely found in aquatic environment, from Erlenmeyer flask to bioreactor by the white-rot fungus Trametes versicolor. In Erlenmeyer flask, CBZ at approximately 9mgL-1 was almost completely eliminated (94%) after 6d, while at near environmentally relevant concentrations of 50μgL-1, 61% of the contaminant was degraded in 7d. Acridone, acridine, 10,11-dihydro-10,11-dihydroxy-CBZ, and 10, 11-epoxy-CBZ were identified as major metabolites, confirming the degradation of CBZ. The degradation process was then carried out in an air pulsed fluidized bioreactor operated in batch and continuous mode. Around 96% of CBZ was removed after 2 days in batch mode operation, and 10,11-dihydro-10,11-epoxycarbamazepine was found as unique metabolite. In bioreactor operated in continuous mode with a hydraulic retention time of 3d, 54% of the inflow concentration (approx. 200μgL-1) was reduced at the steady state (25d) with a CBZ degradation rate of 11.9μgCBZg-1 dry weight d-1. No metabolite was detected in the culture broth. Acute toxicity tests (Microtox) indicated that the final culture broth in both batch and continuous mode operation were non toxic, with 15min EC50 values of 24% and 77%, respectively. © 2011 Elsevier Ltd.
KW - Bioreactor
KW - Carbamazepine
KW - Continuous treatment
KW - Pharmaceuticals
KW - Trametes versicolor
U2 - https://doi.org/10.1016/j.watres.2011.11.063
DO - https://doi.org/10.1016/j.watres.2011.11.063
M3 - Article
VL - 46
SP - 955
EP - 964
JO - Water Research
JF - Water Research
SN - 0043-1354
ER -