Dechlorination of 1,2,3- and 1,2,4-trichlorobenzene by the white-rot fungus Trametes versicolor

Ernest Marco-Urrea, Miriam Pérez-Trujillo, Gloria Caminal, Teresa Vicent

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    28 Citations (Scopus)

    Abstract

    The degradation of 1,2,3-, 1,3,5- and 1,2,4-trichlorobenzene (TCB) by the white-rot fungus Trametes versicolor was studied. Time course experiments showed a degradation rate of 2.27 and 2.49 nmol d-1 mg-1 dry weight of biomass during the first 4 d of incubation in cultures spiked with 6 mg L-1 of 1,2,3- and 1,2,4-TCB, respectively. A high percent of degradation of 91.1% (1,2,3-TCB) and 79.6 (1,2,4-TCB) was obtained after 7 d. However, T. versicolor was not able to degrade 1,3,5-TCB under the conditions tested. For a range of concentrations of 1,2,4-TCB between 6.5 and 23 mg L-1, a complete dechlorination of the molecule was observed. Cytochrome P450 monooxygenase appears to be involve in the first step of 1,2,4-TCB degradation, as evidenced by marked inhibition of both dechlorination and degradation of 1,2,4-TCB in the presence of the known cyt P450 inhibitors 1-aminobenzotriazole and piperonyl butoxide. Four intermediates formed from 1,2,4-TCB degradation were detected the second day of incubation, which did not appear the seventh day: 2,3,5-trichloromuconate, its corresponding carboxymethylenebutenolide, 2- or 5-chloro-4-oxo-2-hexendioic acid and 2- or 5-chloro-5-hydroxy-4-oxo-2-pentenoic acid. Based on these results, a degradation pathway of 1,2,4-TCB through cyt P450 monooxygenase and epoxide hydrolase was proposed. © 2009 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)1141-1147
    JournalJournal of Hazardous Materials
    Volume166
    Issue number2-3
    DOIs
    Publication statusPublished - 30 Jul 2009

    Keywords

    • Biodegradation
    • Cytochrome P450
    • Trametes versicolor
    • Trichlorobenzene
    • White-rot fungi

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