TY - JOUR
T1 - Dechlorination of 1,2,3- and 1,2,4-trichlorobenzene by the white-rot fungus Trametes versicolor
AU - Marco-Urrea, Ernest
AU - Pérez-Trujillo, Miriam
AU - Caminal, Gloria
AU - Vicent, Teresa
PY - 2009/7/30
Y1 - 2009/7/30
N2 - The degradation of 1,2,3-, 1,3,5- and 1,2,4-trichlorobenzene (TCB) by the white-rot fungus Trametes versicolor was studied. Time course experiments showed a degradation rate of 2.27 and 2.49 nmol d-1 mg-1 dry weight of biomass during the first 4 d of incubation in cultures spiked with 6 mg L-1 of 1,2,3- and 1,2,4-TCB, respectively. A high percent of degradation of 91.1% (1,2,3-TCB) and 79.6 (1,2,4-TCB) was obtained after 7 d. However, T. versicolor was not able to degrade 1,3,5-TCB under the conditions tested. For a range of concentrations of 1,2,4-TCB between 6.5 and 23 mg L-1, a complete dechlorination of the molecule was observed. Cytochrome P450 monooxygenase appears to be involve in the first step of 1,2,4-TCB degradation, as evidenced by marked inhibition of both dechlorination and degradation of 1,2,4-TCB in the presence of the known cyt P450 inhibitors 1-aminobenzotriazole and piperonyl butoxide. Four intermediates formed from 1,2,4-TCB degradation were detected the second day of incubation, which did not appear the seventh day: 2,3,5-trichloromuconate, its corresponding carboxymethylenebutenolide, 2- or 5-chloro-4-oxo-2-hexendioic acid and 2- or 5-chloro-5-hydroxy-4-oxo-2-pentenoic acid. Based on these results, a degradation pathway of 1,2,4-TCB through cyt P450 monooxygenase and epoxide hydrolase was proposed. © 2009 Elsevier B.V. All rights reserved.
AB - The degradation of 1,2,3-, 1,3,5- and 1,2,4-trichlorobenzene (TCB) by the white-rot fungus Trametes versicolor was studied. Time course experiments showed a degradation rate of 2.27 and 2.49 nmol d-1 mg-1 dry weight of biomass during the first 4 d of incubation in cultures spiked with 6 mg L-1 of 1,2,3- and 1,2,4-TCB, respectively. A high percent of degradation of 91.1% (1,2,3-TCB) and 79.6 (1,2,4-TCB) was obtained after 7 d. However, T. versicolor was not able to degrade 1,3,5-TCB under the conditions tested. For a range of concentrations of 1,2,4-TCB between 6.5 and 23 mg L-1, a complete dechlorination of the molecule was observed. Cytochrome P450 monooxygenase appears to be involve in the first step of 1,2,4-TCB degradation, as evidenced by marked inhibition of both dechlorination and degradation of 1,2,4-TCB in the presence of the known cyt P450 inhibitors 1-aminobenzotriazole and piperonyl butoxide. Four intermediates formed from 1,2,4-TCB degradation were detected the second day of incubation, which did not appear the seventh day: 2,3,5-trichloromuconate, its corresponding carboxymethylenebutenolide, 2- or 5-chloro-4-oxo-2-hexendioic acid and 2- or 5-chloro-5-hydroxy-4-oxo-2-pentenoic acid. Based on these results, a degradation pathway of 1,2,4-TCB through cyt P450 monooxygenase and epoxide hydrolase was proposed. © 2009 Elsevier B.V. All rights reserved.
KW - Biodegradation
KW - Cytochrome P450
KW - Trametes versicolor
KW - Trichlorobenzene
KW - White-rot fungi
U2 - 10.1016/j.jhazmat.2008.12.076
DO - 10.1016/j.jhazmat.2008.12.076
M3 - Article
VL - 166
SP - 1141
EP - 1147
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
IS - 2-3
ER -