Cytokine production in patients with cirrhosis and TLR4 polymorphisms

Juan Camilo Nieto, Elisabet Sánchez, Eva Román, Silvia Vidal, Laia Oliva, Carlos Guarner-Argente, Maria Poca, Xavier Torras, Cándido Juárez, Carlos Guarner, German Soriano

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13 Citations (Scopus)


© 2014 Baishideng Publishing Group Inc. All rights reserved. AIM: To analyze the cytokine production by peripheral blood cells from cirrhotic patients with and without TLR4 D299G and/or T399I polymorphisms.METHODS: The study included nine patients with cirrhosis and TLR4 D299G and/or T399I polymorphisms, and 10 wild-type patients matched for age, sex and degree of liver failure. TLR4 polymorphisms were determined by sequence-based genotyping. Cytokine production by peripheral blood cells was assessed spontaneously and also after lipopolysaccharide (LPS) and lipoteichoic acid (LTA) stimulation.RESULTS: Patients with TLR4 polymorphisms had a higher incidence of previous hepatic encephalopathy than wild-type patients (78% vs 20%, P = 0.02). Spontaneous production of interleukin (IL)-6 and IL-10 was lower in patients with TLR4 polymorphisms than in wild-type patients [IL-6: 888.7 (172.0-2119.3) pg/mL vs 5540.4 (1159.2-26053.9) pg/mL, P < 0.001; IL-10: 28.7 (6.5-177.1) pg/mL vs 117.8 (6.5-318.1) pg/mL, P = 0.02]. However, the production of tumor necrosis factor-α, IL-6 and IL-10 after LPS and LTA stimulation was similar in the two groups.CONCLUSION: TLR4 polymorphisms were associated with a distinctive pattern of cytokine production in cirrhotic patients, suggesting that they play a role in the development of cirrhosis complications.
Original languageEnglish
Pages (from-to)17516-17524
JournalWorld Journal of Gastroenterology
Issue number46
Publication statusPublished - 14 Dec 2014


  • Genetic factors
  • Hepatic encephalopathy
  • Infections
  • Inflammatory response


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