We have characterized the expression of six cytokine mRNAs in highly purified B cells from bovine leukemia virus (BLV)-infected cows with persistent lymphocytosis. Selected cytokine mRNAs included those encoding tumor necrosis factor (TNF), lymphotoxin-α (LT-α), transforming growth factor-β 1 (TGF-β 1), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-10 (IL-10). Fresh B cells from cows with persistent lymphocytosis constitutively transcribed TNF, LT-α and TGF-β 1 mRNAs. Although IL-1β, IL-6 and IL-10 mRNAs were barely detectable in fresh B cells from cows with persistent lymphocytosis, transcripts encoding these cytokines were strongly and rapidly upregulated in B cells after cell culture. Results from this study provide the first evidence that B cells infected with BLV express specific cytokine mRNAs in vivo. © 2004 Elsevier Ltd. All rights reserved.
|Publication status||Published - 7 Oct 2004|
- B cell
- Bovine leukemia virus
- Persistent lymphocytosis