Cytogenetic sexing of mouse embryos

Carles Giménez, Josep Egozcue, Francesca Vidal

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Cytogenetic sexing by means of chromosome C-banding was carried out in 1/2 and 2/4 biopsied mouse embryos. Chromosome spreads were obtained in 85.0% of 1/2-embryos and in 77.5% of 2/4-embryos. Sexing was accomplished in 30.0% of 1/2-embryos and 52.5% of 2/4-embryos, with a loss due to poor technique of 12.5 and 15.0% respectively. Transfer of sexed embryos resulted in a low implantation rate. The two live fetuses obtained were of the predicted sex. © 1993 Oxford University Press.
Original languageEnglish
Pages (from-to)470-474
JournalHuman Reproduction
Volume8
Issue number3
DOIs
Publication statusPublished - 1 Jan 1993

Keywords

  • Biopsy
  • Blastomeres
  • C-banding
  • Cytogenetics
  • Preimplantation diagnosis

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