A sequential fluorescence in situ hybridization (FISH) technique is described. This method allows the detection of up to eighteen chromosome pairs in consecutive hybridizations (8 steps) on the same metaphase using centromeric, whole chromosome painting, and single copy DNA probes with different fluorochromes. The technique may be used with diagnostic purposes in cases with poor cytogenetic material.
|Journal||Cancer Genetics and Cytogenetics|
|Publication status||Published - 15 Jan 1998|
Escudero, T., Fuster, C., Coll, M. D., & Egozcue, J. (1998). Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization. Cancer Genetics and Cytogenetics, 100, 111-113. https://doi.org/10.1016/S0165-4608(97)00029-0