Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization

Tomás Escudero, Carmen Fuster, M. Dolors Coll, José Egozcue

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    8 Citations (Scopus)

    Abstract

    A sequential fluorescence in situ hybridization (FISH) technique is described. This method allows the detection of up to eighteen chromosome pairs in consecutive hybridizations (8 steps) on the same metaphase using centromeric, whole chromosome painting, and single copy DNA probes with different fluorochromes. The technique may be used with diagnostic purposes in cases with poor cytogenetic material.
    Original languageEnglish
    Pages (from-to)111-113
    JournalCancer Genetics and Cytogenetics
    Volume100
    DOIs
    Publication statusPublished - 15 Jan 1998

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    Escudero, T., Fuster, C., Coll, M. D., & Egozcue, J. (1998). Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization. Cancer Genetics and Cytogenetics, 100, 111-113. https://doi.org/10.1016/S0165-4608(97)00029-0