CYP2D6 and CYP2A6 biotransform dietary tyrosol into hydroxytyrosol

Jose Rodríguez-Morató, Patricia Robledo, Julie Anne Tanner, Anna Boronat, Clara Pérez-Mañá, C. Y. Oliver Chen, Rachel F. Tyndale, Rafael de la Torre

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

© 2016 Elsevier Ltd The dietary phenol tyrosol has been reported to be endogenously transformed into hydroxytyrosol, a potent antioxidant with multiple health benefits. In this work, we evaluated whether tyrosine hydroxylase (TH) and cytochrome P450s (CYPs) catalyzed this process. To assess TH involvement, Wistar rats were treated with α-methyl-L-tyrosine and tyrosol. Tyrosol was converted into hydroxytyrosol whilst α-methyl-L-tyrosine did not inhibit the biotransformation. The role of CYP was assessed in human liver microsomes (HLM) and tyrosol-to-hydroxytyrosol conversion was observed. Screening with selective enzymatic CYP inhibitors identified CYP2A6 as the major isoform involved in this process. Studies with baculosomes further demonstrated that CYP2D6 and CYP3A4 could transform tyrosol into hydroxytyrosol. Experiments using human genotyped livers showed an interindividual variability in hydroxytyrosol formation and supported findings that CYP2D6 and CYP2A6 mediated this reaction. The dietary health benefits of tyrosol-containing foods remain to be evaluated in light of CYP pharmacogenetics.
Original languageEnglish
Pages (from-to)716-725
JournalFood Chemistry
Volume217
DOIs
Publication statusPublished - 15 Feb 2017

Keywords

  • CYP2A6
  • CYP2D6
  • Human liver microsomes
  • Hydroxytyrosol
  • Metabolism
  • Tyrosol

Fingerprint

Dive into the research topics of 'CYP2D6 and CYP2A6 biotransform dietary tyrosol into hydroxytyrosol'. Together they form a unique fingerprint.

Cite this