CXCR4+-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis

Olivia Cano-Garrido, María Virtudes Céspedes, Ugutz Unzueta, Paolo Saccardo, Mònica Roldán, Alejandro Sánchez-Chardi, Rafael Cubarsi, Esther Vázquez, Ramon Mangues, Elena García-Fruitós, Antonio Villaverde

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

© 2016 A Villaverde. Aim: Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials. Materials & methods: Biophysical properties, cell penetrability and in vivo biodistribution upon systemic administration of tumor-targeted protein nanoparticles produced in L. lactis have been compared with the equivalent material produced in E. coli. Results: Protein nanoparticles have been efficiently produced in L. lactis, showing the desired size, internalization properties and biodistribution. Conclusion: In vitro and in vivo data confirm the potential and robustness of the production platform, pointing out L. lactis as a fascinating cell factory for the biofabrication of protein materials intended for therapeutic applications.
Original languageEnglish
Pages (from-to)2387-2398
JournalNanomedicine
Volume11
Issue number18
DOIs
Publication statusPublished - 1 Sep 2016

Keywords

  • protein nanoparticles
  • recombinant proteins
  • tumor-homing peptides

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