TY - JOUR
T1 - CXCR4+-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis
AU - Cano-Garrido, Olivia
AU - Céspedes, María Virtudes
AU - Unzueta, Ugutz
AU - Saccardo, Paolo
AU - Roldán, Mònica
AU - Sánchez-Chardi, Alejandro
AU - Cubarsi, Rafael
AU - Vázquez, Esther
AU - Mangues, Ramon
AU - García-Fruitós, Elena
AU - Villaverde, Antonio
PY - 2016/9/1
Y1 - 2016/9/1
N2 - © 2016 A Villaverde. Aim: Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials. Materials & methods: Biophysical properties, cell penetrability and in vivo biodistribution upon systemic administration of tumor-targeted protein nanoparticles produced in L. lactis have been compared with the equivalent material produced in E. coli. Results: Protein nanoparticles have been efficiently produced in L. lactis, showing the desired size, internalization properties and biodistribution. Conclusion: In vitro and in vivo data confirm the potential and robustness of the production platform, pointing out L. lactis as a fascinating cell factory for the biofabrication of protein materials intended for therapeutic applications.
AB - © 2016 A Villaverde. Aim: Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials. Materials & methods: Biophysical properties, cell penetrability and in vivo biodistribution upon systemic administration of tumor-targeted protein nanoparticles produced in L. lactis have been compared with the equivalent material produced in E. coli. Results: Protein nanoparticles have been efficiently produced in L. lactis, showing the desired size, internalization properties and biodistribution. Conclusion: In vitro and in vivo data confirm the potential and robustness of the production platform, pointing out L. lactis as a fascinating cell factory for the biofabrication of protein materials intended for therapeutic applications.
KW - protein nanoparticles
KW - recombinant proteins
KW - tumor-homing peptides
U2 - https://doi.org/10.2217/nnm-2016-0200
DO - https://doi.org/10.2217/nnm-2016-0200
M3 - Article
VL - 11
SP - 2387
EP - 2398
JO - Nanomedicine
JF - Nanomedicine
SN - 1743-5889
IS - 18
ER -