Cutting at the right place - The importance of selective limited proteolysis in the activation of proproteinase E

F. Xavier Gomis-Rüth, Mariola Gómez-Ortiz, Josep Vendrell, Salvador Ventura, Wolfram Bode, Robert Huber, Francesc X. Avilés

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Proteinase E is a proteolytic enzyme which belongs to a distinct subfamily of chymotrypsin-like serine endopeptidases. Its proform from the bovine pancreatic system has been structurally analyzed by X-ray crystallography for the intact native form, with a 11-residue N-terminal activation peptide, in a ternary complex with chymotrypsinogen C and procarboxypeptidase A [Gomis-Ruth, F. X., Gomez, M., Bode, W., Huber, R. and Aviles, F. X. (1995) The three-dimensional structure of the native ternary complex of bovine pancreatic procarboxypeptidase A with proproteinase E and chymotrypsinogen C, EMBO J. 14, 4387-4394]. Also for a N-terminally truncated form, lacking the first 13 residues and called subunit III, a crystal structure is available [Pignol, D., Gaboriaud, C., Michon, T, Kerfelec, B., Chapus, C. and Fontecilia-Camps, J. C. (1994) Crystal structure of bovine procarboxypeptidase A-S6 subunit III, a highly structured truncated zymogen E, EMBO J. 8, 1763-1771]. Both structures are well defined by electron density, except for the first 7 residues of subunit III. However, both structures present large deviations of up to 2 nm in several regions, indicating that they correspond to two quite distinct states of low free energy, influenced by very few contacts made via the N-terminal segment. As no structure of an active proteinase E is known so far, pancreatic porcine elastase has been chosen as a model for this enzyme and an activation mechanism for this distinct serine endopeptidase subfamily is proposed.
Original languageEnglish
Pages (from-to)839-844
JournalEuropean Journal of Biochemistry
Volume251
DOIs
Publication statusPublished - 1 Feb 1998

Keywords

  • Activation mechanism
  • Elastase
  • Proproteinase E
  • Serine
  • X-ray crystal structure

Fingerprint Dive into the research topics of 'Cutting at the right place - The importance of selective limited proteolysis in the activation of proproteinase E'. Together they form a unique fingerprint.

Cite this