Crystal structure of Bacillus licheniformis 1,3-1,4-β-d-glucan 4-glucanohydrolase at 1.8 Å resolution

Michael Hahn, Jaume Pons, Antoni Planas, Enrique Querol, Udo Heinemann

Research output: Contribution to journalArticleResearchpeer-review

73 Citations (Scopus)

Abstract

The crystal structure of the 1,3-1,4-β-d-glucan 4-glucanohydrolase from Bacillus licheniformis is solved at a resolution of 1.8 Å and refined to R = 16.5%. The protein has a similar β-sandwich structure as the homologous enzyme from Bacillus macerans and the hybrid H(A16-M). This demonstrates that the jellyroll fold of these proteins is remarkably rigid and only weakly influenced by crystal contacts. The crystal structure permits to extend mechanistic considerations derived for the B. licheniformis enzyme to the entire class of bacterial 1,3-1,4-β-d-glucan 4-glucanohydrolases. © 1995.
Original languageEnglish
Pages (from-to)221-224
JournalFEBS Letters
Volume374
Issue number2
DOIs
Publication statusPublished - 30 Oct 1995

Keywords

  • 1,3-1,4-β-d-Glucan 4-glucanohydrolase
  • 1,3-1,4-β-Glucanase
  • Bacillus licheniformis
  • Crystal structure
  • Enzyme mechanism
  • β-Glucan hydrolysis

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