Coxiella burnetii total immunoglobulin G, phase I and phase II immunoglobulin G antibodies, and bacterial shedding in young dams in persistently infected dairy herds

Beatriz Serrano-Pérez, Sonia Almería, Joan Tutusaus, Isabel Jado, Pedro Anda, Eva Monleón, Juan Badiola, Irina Garcia-Ispierto, Fernando López-Gatius

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2 Citations (Scopus)

Abstract

© 2015 The Author(s) The current study examines Coxiella burnetii infection patterns in young dairy dams around the calving period in persistently infected high-producing dairy herds. Infection patterns were determined in terms of total immunoglobulin G (IgG) and phase-specific IgG antibodies by enzyme-linked immunosorbent assay and bacterial shedding by real-time polymerase chain reaction (qPCR). On days 171–177 of gestation, at parturition, and on days 15–21 and 91–97 postpartum, 7 first-parity cows and 7 second-parity cows were sampled for serology and qPCR. Total phase-specific I (PhI) and II (PhII) IgG antibodies were detected in 2 animals at days 171–177 of gestation. Four additional animals underwent seroconversion on days 91–97 postpartum. Three of 6 seropositive dams according to total IgG, showed a PhI+/PhII+ profile, whereas dams that seroconverted exhibited a PhI–/PhII+ (2/6) or PhI+/PhII– (1/6) profile. An indirect fluorescent antibody test for PhI and PhII immunoglobulin M (IgM) was performed on plasma samples from the shedding dams, confirming seropositivity in a first-parity dam that seroconverted, and detecting a sudden spike of PhI-IgM antibodies in 1 further dam. No relationship was detected in young C. burnetii–infected animals between total IgG, PhI and/or PhII antibodies, and bacterial shedding throughout the study period. The highest bacterial load measured by qPCR was recorded in a second-parity dam. This animal presented abnormal peripheral blood counts, which would be an indication of severe peripheral blood alterations in some infected cattle. This study suggests that young shedder cows are mostly seronegative in early stages of infection.
Original languageEnglish
Pages (from-to)167-176
JournalJournal of Veterinary Diagnostic Investigation
Volume27
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Cattle
  • Q fever
  • hematology
  • phase-specific antibodies
  • polymerase chain reaction

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