Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS

S. Mateo, F. Vidal, M. Parriego, I. Rodríguez, V. Montalvo, A. Veiga, M. Boada

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17 Citations (Scopus)

Abstract

© 2017, Springer Science+Business Media New York. Purpose: The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage. Methods: Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012–December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator. Embryo biopsy was performed on day 3 and genetic analysis approached by array comparative genomic hybridization. Results: Chromosomal analysis revealed that 17% (15/88) of embryos derived from 1PN 2PB zygotes were diagnosed as euploid. After blastomere biopsy at day 3, the blastocyst rate at day 5 was 3.4% (3/88). Only 2.3% (2/88) euploid blastocysts were obtained. In two couples and after counseling and patient agreement, the transfer of a euploid blastocyst from a 1PN 2PB ICSI zygote was performed resulting in the birth of a healthy child. Conclusions: These results open the possibility to consider embryos coming from 1PN 2PB ICSI zygotes for transfer when no other embryos from 2PN 2PB ICSI zygotes are available and if a PGS diagnosis of euploidy is obtained. Confirmation of biparental inheritance is strongly recommended.
Original languageEnglish
Pages (from-to)905-911
JournalJournal of Assisted Reproduction and Genetics
Volume34
Issue number7
DOIs
Publication statusPublished - 1 Jul 2017

Keywords

  • 1PN
  • Monopronucleated zygotes
  • PGS
  • Time-lapse
  • aCGH

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