Continuous measurement of acute toxicity in water using a solid state microrespirometer

Fco Javier Del Campo, Olga Ordeig, Nuria Vigués, Neus Godino, Jordi Mas, Francesc Xavier Muñoz

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)


This work presents the first true solid-state microrespirometer. It consists on a naturally developed biofilm of Pseudomonas aeruginosa over a Nafion modified array of gold microdisc electrodes. Such a device can be used to monitor acute toxicity in water streams based on the oxygen reduction current recorded at the microelectrode array. When a healthy biofilm is exposed to a toxic stream the bacteria conforming stop breathing which results in an immediate increase of the oxygen supply reaching the microelectrode array. The fabrication and use of such a microrespirometer is described, and a possible operation mode for a real application is presented. Given that different waters may contain toxic compounds of diverse nature, the respirometer presented here provides qualitative information only. © 2007 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)515-521
JournalSensors and Actuators, B: Chemical
Publication statusPublished - 1 Oct 2007


  • Biofilm
  • Linear sweep voltammetry
  • Microrespirometry
  • Oxygen reduction
  • Ultramicroelectrode arrays


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