This work presents the first true solid-state microrespirometer. It consists on a naturally developed biofilm of Pseudomonas aeruginosa over a Nafion modified array of gold microdisc electrodes. Such a device can be used to monitor acute toxicity in water streams based on the oxygen reduction current recorded at the microelectrode array. When a healthy biofilm is exposed to a toxic stream the bacteria conforming stop breathing which results in an immediate increase of the oxygen supply reaching the microelectrode array. The fabrication and use of such a microrespirometer is described, and a possible operation mode for a real application is presented. Given that different waters may contain toxic compounds of diverse nature, the respirometer presented here provides qualitative information only. © 2007 Elsevier B.V. All rights reserved.
|Journal||Sensors and Actuators, B: Chemical|
|Publication status||Published - 1 Oct 2007|
- Linear sweep voltammetry
- Oxygen reduction
- Ultramicroelectrode arrays