Constitutive Cyclin O deficiency results in penetrant hydrocephalus, impaired growth and infertility

Marc Núnez-Ollé, Carole Jung, Berta Terré, Norman A. Balsiger, Cristina Plata, Ramon Roset, Carlos Pardo-Pastor, Marta Garrido, Santiago Rojas, Francesc Alameda, Josep Lloreta, Juan Martín-Caballero, Juana M. Flores, Travis H. Stracker, Miguel A. Valverde, Francisco J. Muñoz, Gabriel Gil-Gómez

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12 Citations (Scopus)

Abstract

© Núnez-Ollé et al. Cyclin O (encoded by CCNO) is a member of the cyclin family with regulatory functions in ciliogenesis and apoptosis. Homozygous CCNO mutations have been identified in human patients with Reduced Generation of Multiple Motile Cilia (RGMC) and conditional inactivation of Ccno in the mouse recapitulates some of the pathologies associated with the human disease. These include defects in the development of motile cilia and hydrocephalus. To further investigate the functions of Ccno in vivo, we have generated a new mouse model characterized by the constitutive loss of Ccno in all tissues and followed a cohort during ageing. Ccno-/- mice were growth impaired and developed hydrocephalus with high penetrance. In addition, some Ccno+/- mice also developed hydrocephalus and affected Ccno-/- and Ccno+/- mice exhibited additional CNS defects including cortical thinning and hippocampal abnormalities. In addition to the CNS defects, both male and female Ccno-/- mice were infertile and female mice exhibited few motile cilia in the oviduct. Our results further establish CCNO as an important gene for normal development and suggest that heterozygous CCNO mutations could underlie hydrocephalus or diminished fertility in some human patients.
Original languageEnglish
Pages (from-to)99261-99273
JournalOncotarget
Volume8
Issue number59
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Ciliogenesis
  • Cyclin O
  • Development
  • Gerotarget
  • Hydrocephalus
  • Neurogenesis

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    Núnez-Ollé, M., Jung, C., Terré, B., Balsiger, N. A., Plata, C., Roset, R., Pardo-Pastor, C., Garrido, M., Rojas, S., Alameda, F., Lloreta, J., Martín-Caballero, J., Flores, J. M., Stracker, T. H., Valverde, M. A., Muñoz, F. J., & Gil-Gómez, G. (2017). Constitutive Cyclin O deficiency results in penetrant hydrocephalus, impaired growth and infertility. Oncotarget, 8(59), 99261-99273. https://doi.org/10.18632/oncotarget.21818