Consolidated membrane technology, consisting of a simple compression of dry components, is proposed for production of potentiometric biosensor membranes. In order to present this concept, two different kinds of conventional potentiometric biosensors have been designed both based on this novel biocomposite membrane technology. In the first case, a D-amygdalin biosensor based on β-glucosidase incorporated in an iodide-selective membrane has been prepared. In the second case, two enzymes, glucose oxidase (GOD) and peroxidase (POD) are incorporated in the iodide-selective membrane to form a biocomposite for glucose sensing. Some of the main characteristics of the developed potentiometric biosensors are discussed. This involves both the manufacturing technology and the analytical evaluation of biosensors. Results obtained, indicate that this pressing technology is suitable for mass production of potentiometric biosensing devices. For the biosensor based on β-glucosidase, the recommended pressure was 2.3·104 kg/cm2. At this pressure, it was observed that the immobilization of enzymes in the bulk of the electroactive salts was efficient. The D-amygdalin biosensor had a linear response in the range of 3.9-10-3 to 10-2 M and a practical lower detection limit of 2.5·10-3 M. For the biosensor based on GOD and POD the optimal consolidation pressure was 2.0·104 kg/cm2. In batch measurements, this biosensor has a response time of approximately 2 min. The linear range was between 0.3 and 1.1 mM glucose.
|Journal||Sensors and Actuators, B: Chemical|
|Publication status||Published - 23 Nov 1999|