Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

Eshan Ghosh; Hemlata Dwivedi; Mithu Baidya; Ashish Srivastava; Punita Kumari; Tomek Stepniewski; Hee Ryung Kim; Mi Hye Lee; Jaana van Gastel; Madhu Chaturvedi; Debarati Roy; Shubhi Pandey; Jagannath Maharana; Ramon Guixà-González; Louis M. Luttrell; Ka Young Chung; Somnath Dutta; Jana Selent; Arun K. Shukla

doi:https://doi.org/10.1016/j.celrep.2019.08.053

Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

Eshan Ghosh, Hemlata Dwivedi, Mithu Baidya, Ashish Srivastava, Punita Kumari, Tomek Stepniewski, Hee Ryung Kim, Mi Hye Lee, Jaana van Gastel, Madhu Chaturvedi, Debarati Roy, Shubhi Pandey, Jagannath Maharana, Ramon Guixà-González, Louis M. Luttrell, Ka Young Chung, Somnath Dutta, Jana Selent, Arun K. Shukla

Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine and Public Health

Research output: Contribution to journal › Article › Research

33 Citations (Scopus)

Abstract

© 2019 The Author(s) Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.

Original language	English
Pages (from-to)	3287-3299.e6
Journal	Cell Reports
Volume	28
DOIs	https://doi.org/10.1016/j.celrep.2019.08.053
Publication status	Published - 24 Sept 2019

Keywords

GPCRs
antibody fragments
biased agonism
biosensors
cellular signaling
desensitization
electron microscopy
negative staining
β-arrestins

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https://doi.org/10.1016/j.celrep.2019.08.053

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Ghosh, E., Dwivedi, H., Baidya, M., Srivastava, A., Kumari, P., Stepniewski, T., Kim, H. R., Lee, M. H., van Gastel, J., Chaturvedi, M., Roy, D., Pandey, S., Maharana, J., Guixà-González, R., Luttrell, L. M., Chung, K. Y., Dutta, S., Selent, J., & Shukla, A. K. (2019). Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms. Cell Reports, 28, 3287-3299.e6. https://doi.org/10.1016/j.celrep.2019.08.053

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title = "Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms",

abstract = "{\textcopyright} 2019 The Author(s) Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.",

keywords = "GPCRs, antibody fragments, biased agonism, biosensors, cellular signaling, desensitization, electron microscopy, negative staining, β-arrestins",

author = "Eshan Ghosh and Hemlata Dwivedi and Mithu Baidya and Ashish Srivastava and Punita Kumari and Tomek Stepniewski and Kim, {Hee Ryung} and Lee, {Mi Hye} and {van Gastel}, Jaana and Madhu Chaturvedi and Debarati Roy and Shubhi Pandey and Jagannath Maharana and Ramon Guix{\`a}-Gonz{\'a}lez and Luttrell, {Louis M.} and Chung, {Ka Young} and Somnath Dutta and Jana Selent and Shukla, {Arun K.}",

year = "2019",

month = sep,

day = "24",

doi = "https://doi.org/10.1016/j.celrep.2019.08.053",

language = "English",

volume = "28",

pages = "3287--3299.e6",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

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Ghosh, E, Dwivedi, H, Baidya, M, Srivastava, A, Kumari, P, Stepniewski, T, Kim, HR, Lee, MH, van Gastel, J, Chaturvedi, M, Roy, D, Pandey, S, Maharana, J, Guixà-González, R, Luttrell, LM, Chung, KY, Dutta, S, Selent, J & Shukla, AK 2019, 'Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms', Cell Reports, vol. 28, pp. 3287-3299.e6. https://doi.org/10.1016/j.celrep.2019.08.053

TY - JOUR

T1 - Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

AU - Ghosh, Eshan

AU - Dwivedi, Hemlata

AU - Baidya, Mithu

AU - Srivastava, Ashish

AU - Kumari, Punita

AU - Stepniewski, Tomek

AU - Kim, Hee Ryung

AU - Lee, Mi Hye

AU - van Gastel, Jaana

AU - Chaturvedi, Madhu

AU - Roy, Debarati

AU - Pandey, Shubhi

AU - Maharana, Jagannath

AU - Guixà-González, Ramon

AU - Luttrell, Louis M.

AU - Chung, Ka Young

AU - Dutta, Somnath

AU - Selent, Jana

AU - Shukla, Arun K.

PY - 2019/9/24

Y1 - 2019/9/24

N2 - © 2019 The Author(s) Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.

AB - © 2019 The Author(s) Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.

KW - GPCRs

KW - antibody fragments

KW - biased agonism

KW - biosensors

KW - cellular signaling

KW - desensitization

KW - electron microscopy

KW - negative staining

KW - β-arrestins

U2 - https://doi.org/10.1016/j.celrep.2019.08.053

DO - https://doi.org/10.1016/j.celrep.2019.08.053

M3 - Article

C2 - 31553900

SN - 2211-1247

VL - 28

SP - 3287-3299.e6

JO - Cell Reports

JF - Cell Reports

ER -

Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

Abstract

Keywords

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