Confocal laser scanning microscopy as a tool to determine cyanobacteria biomass in microbial mats

A. Solé, N. Gaju, S. Méndez-Álvarez, I. Esteve

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Abstract

We have developed a method based on confocal laser scanning microscopy for detection and quantification of cyanobacteria from the Ebro Delta microbial mats. Cyanobacteria play a major role as primary producers in microbial mats; it is difficult, however, to apply classical methods to estimate their biomass because they establish strong interactions with detritic particles. The protocol described here allows the localization of individual cells or filaments with micrometre precision without the need to either manipulate or stain the samples. This method is suitable for studying biomass 'in situ' in microbial mats.
Original languageEnglish
Pages (from-to)258-262
JournalJournal of Microscopy
Volume204
Issue number3
DOIs
Publication statusPublished - 1 Dec 2001

Keywords

  • Biomass
  • Confocal laser scanning microscopy
  • Cyanobacteria
  • Microbial mats

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