Abstract
Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r2=0.992) for low spore concentrations ranging from 1 to 100CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. © 2010 Elsevier B.V.
Original language | English |
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Pages (from-to) | 2309-2312 |
Journal | Biosensors and Bioelectronics |
Volume | 25 |
Issue number | 10 |
DOIs | |
Publication status | Published - 1 Jun 2010 |
Keywords
- Bacillus sp.
- Biosensor
- Bioterrorism
- Nanowire
- Polypyrrole
- Spore detection