Conducting polymer nanowire-based chemiresistive biosensor for the detection of bacterial spores

Cristina García-Aljaro, Mangesh A. Bangar, Eva Baldrich, Francisco Javier Muñoz, Ashok Mulchandani

    Research output: Contribution to journalArticleResearchpeer-review

    38 Citations (Scopus)

    Abstract

    Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r2=0.992) for low spore concentrations ranging from 1 to 100CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. © 2010 Elsevier B.V.
    Original languageEnglish
    Pages (from-to)2309-2312
    JournalBiosensors and Bioelectronics
    Volume25
    Issue number10
    DOIs
    Publication statusPublished - 1 Jun 2010

    Keywords

    • Bacillus sp.
    • Biosensor
    • Bioterrorism
    • Nanowire
    • Polypyrrole
    • Spore detection

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