Conducting polymer nanowire-based chemiresistive biosensor for the detection of bacterial spores

Cristina García-Aljaro; Mangesh A. Bangar; Eva Baldrich; Francisco Javier Muñoz; Ashok Mulchandani

doi:10.1016/j.bios.2010.03.021

Conducting polymer nanowire-based chemiresistive biosensor for the detection of bacterial spores

Cristina García-Aljaro, Mangesh A. Bangar, Eva Baldrich, Francisco Javier Muñoz, Ashok Mulchandani

Research output: Contribution to journal › Article › Research › peer-review

37 Citations (Scopus)

Abstract

Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r2=0.992) for low spore concentrations ranging from 1 to 100CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. © 2010 Elsevier B.V.

Original language	English
Pages (from-to)	2309-2312
Journal	Biosensors and Bioelectronics
Volume	25
Issue number	10
DOIs	https://doi.org/10.1016/j.bios.2010.03.021
Publication status	Published - 1 Jun 2010

Keywords

Bacillus sp.
Biosensor
Bioterrorism
Nanowire
Polypyrrole
Spore detection

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title = "Conducting polymer nanowire-based chemiresistive biosensor for the detection of bacterial spores",

abstract = "Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r2=0.992) for low spore concentrations ranging from 1 to 100CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. {\textcopyright} 2010 Elsevier B.V.",

keywords = "Bacillus sp., Biosensor, Bioterrorism, Nanowire, Polypyrrole, Spore detection",

author = "Cristina Garc{\'i}a-Aljaro and Bangar, {Mangesh A.} and Eva Baldrich and Mu{\~n}oz, {Francisco Javier} and Ashok Mulchandani",

year = "2010",

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language = "English",

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T1 - Conducting polymer nanowire-based chemiresistive biosensor for the detection of bacterial spores

AU - García-Aljaro, Cristina

AU - Bangar, Mangesh A.

AU - Baldrich, Eva

AU - Muñoz, Francisco Javier

AU - Mulchandani, Ashok

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r2=0.992) for low spore concentrations ranging from 1 to 100CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. © 2010 Elsevier B.V.

AB - Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r2=0.992) for low spore concentrations ranging from 1 to 100CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. © 2010 Elsevier B.V.

KW - Bacillus sp.

KW - Biosensor

KW - Bioterrorism

KW - Nanowire

KW - Polypyrrole

KW - Spore detection

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DO - 10.1016/j.bios.2010.03.021

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