Concentration-dependent protection by ethanol extract of propolis against γ-ray-induced chromosome damage in human blood lymphocytes

A. Montoro, J. F. Barquinero, M. Almonacid, A. Montoro, N. Sebastià, G. Verdù, V. Sahuquillo, J. Serrano, M. Saiz, J. I. Villaescusa, J. M. Soriano

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Radioprotection with natural products may be relevant to the mitigation of ionizing radiation-induced damage in mammalian systems; in this sense, propolis extracts have shown effects such as antioxidant, antitumoral, anti-inflammatory, and immunostimulant. We report for the first time a cytogenetic study to evaluate the radioprotective effect, in vitro, of propolis against radiation-induced chromosomal damage. Lymphocytes were cultured with increasing concentrations of ethanol extract of propolis (EEP), including 20, 40, 120, 250, 500, 750, 1000, and 2000 μg mL-1 and then exposed to 2 Gy γ-rays. A significant and concentration-dependent decrease is observed in the frequency of chromosome aberrations in samples treated with EEP. The protection against the formation of dicentrics was concentration-dependent, with a maximum protection at 120 μg mL-1 of EEP. The observed frequency of dicentrics is described as negative exponential function, indicating that the maximum protectible fraction of dicentrics is approximately 44. Free radical scavenging and antioxidant activities are the mechanisms that these substances use to protect cells from ionizing radiation. Copyright © 2011 A. Montoro et al.
Original languageEnglish
Article number174853
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
Publication statusPublished - 1 Jan 2011

Fingerprint Dive into the research topics of 'Concentration-dependent protection by ethanol extract of propolis against γ-ray-induced chromosome damage in human blood lymphocytes'. Together they form a unique fingerprint.

Cite this