Comprehensive serological analysis of two successive heterologous vaccines against H5N1 avian influenza virus in exotic birds in zoos

Júlia Vergara-Alert, Hugo Fernández-Bellon, Núria Busquets, Gabriel Alcántara, María Delclaux, Bienvenido Pizarro, Celia Sánchez, Azucena Sánchez, Natàlia Majó, Ayub Darji

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9 Citations (Scopus)

Abstract

In 2005, European Commission directive 2005/744/EC allowed controlled vaccination against avian influenza (AI) virus of valuable avian species housed in zoos. In 2006, 15 Spanish zoos and wildlife centers began a vaccination program with a commercial inactivated H5N9 vaccine. Between November 2007 and May 2008, birds from 10 of these centers were vaccinated again with a commercial inactivated H5N3 vaccine. During these campaigns, pre- and postvaccination samples from different bird orders were taken to study the response against AI virus H5 vaccines. Sera prior to vaccinations with both vaccines were examined for the presence of total antibodies against influenza A nucleoprotein (NP) by a commercial competitive enzyme-linked immunosorbent assay (cELISA). Humoral responses to vaccination were evaluated using a hemagglutination inhibition (HI) assay. In some taxonomic orders, both vaccines elicited comparatively high titers of HI antibodies against H5. Interestingly, some orders, such as Psittaciformes, which did not develop HI antibodies to either vaccine formulation when used alone, triggered notable HI antibody production, albeit in low HI titers, when primed with H5N9 and during subsequent boosting with the H5N3 vaccine. Vaccination with successive heterologous vaccines may represent the best alternative to widely protect valuable and/or endangered bird species against highly pathogenic AI virus infection. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)697-706
JournalClinical and Vaccine Immunology
Volume18
Issue number5
DOIs
Publication statusPublished - 1 May 2011

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    Vergara-Alert, J., Fernández-Bellon, H., Busquets, N., Alcántara, G., Delclaux, M., Pizarro, B., Sánchez, C., Sánchez, A., Majó, N., & Darji, A. (2011). Comprehensive serological analysis of two successive heterologous vaccines against H5N1 avian influenza virus in exotic birds in zoos. Clinical and Vaccine Immunology, 18(5), 697-706. https://doi.org/10.1128/CVI.00013-11