Complementary positional proteomics for screening substrates of endo-and exoproteases

Petra Van Damme; An Staes; Silvia Bronsoms; Kenny Helsens; Niklaas Colaert; Evy Timmerman; Francesc X. Aviles; Joël Vandekerckhove; Kris Gevaert

doi:https://doi.org/10.1038/nmeth.1469

Complementary positional proteomics for screening substrates of endo-and exoproteases

Petra Van Damme, An Staes, Silvia Bronsoms, Kenny Helsens, Niklaas Colaert, Evy Timmerman, Francesc X. Aviles, Joël Vandekerckhove, Kris Gevaert

Research output: Contribution to journal › Article › Research › peer-review

90 Citations (Scopus)

Abstract

We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. © 2010 Nature America, Inc. All rights reserved.

Original language	English
Pages (from-to)	512-515
Journal	Nature Methods
Volume	7
DOIs	https://doi.org/10.1038/nmeth.1469
Publication status	Published - 1 Jul 2010

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title = "Complementary positional proteomics for screening substrates of endo-and exoproteases",

abstract = "We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. {\textcopyright} 2010 Nature America, Inc. All rights reserved.",

author = "{Van Damme}, Petra and An Staes and Silvia Bronsoms and Kenny Helsens and Niklaas Colaert and Evy Timmerman and Aviles, {Francesc X.} and Jo{\"e}l Vandekerckhove and Kris Gevaert",

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Complementary positional proteomics for screening substrates of endo-and exoproteases. / Van Damme, Petra; Staes, An; Bronsoms, Silvia; Helsens, Kenny; Colaert, Niklaas; Timmerman, Evy; Aviles, Francesc X.; Vandekerckhove, Joël; Gevaert, Kris.

In: Nature Methods, Vol. 7, 01.07.2010, p. 512-515.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Van Damme, Petra

AU - Staes, An

AU - Bronsoms, Silvia

AU - Helsens, Kenny

AU - Colaert, Niklaas

AU - Timmerman, Evy

AU - Aviles, Francesc X.

AU - Vandekerckhove, Joël

AU - Gevaert, Kris

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U2 - https://doi.org/10.1038/nmeth.1469

DO - https://doi.org/10.1038/nmeth.1469

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