Abstract
We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. © 2010 Nature America, Inc. All rights reserved.
Original language | English |
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Pages (from-to) | 512-515 |
Journal | Nature Methods |
Volume | 7 |
DOIs | |
Publication status | Published - 1 Jul 2010 |