Complementary positional proteomics for screening substrates of endo-and exoproteases

Petra Van Damme, An Staes, Silvia Bronsoms, Kenny Helsens, Niklaas Colaert, Evy Timmerman, Francesc X. Aviles, Joël Vandekerckhove, Kris Gevaert

Research output: Contribution to journalArticleResearchpeer-review

91 Citations (Scopus)


We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. © 2010 Nature America, Inc. All rights reserved.
Original languageEnglish
Pages (from-to)512-515
JournalNature Methods
Publication statusPublished - 1 Jul 2010


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