Nonesterified fatty acids (NEFA) are often measured in the study of nutrition and growth physiology. Commercial kits for the spectrophotometric determination of NEFA usually involve two coupled enzymatic reactions that produce H2O2, that is quantified with peroxidase. Using goat sera, two commercial kits were compared and the precision analyzed, taking into account variability between goats, and between and within samples. In the tests the Wako kit gave higher values and a better within-samples precision than the Randox kit. Nevertheless, both kit tests gave similar precision and the values obtained with the Randox test were slightly more elevated when human sera were analyzed. However, the stability of the prepared reagents is also better with the Wako kit. This fact could be important when a large number of samples has to be determined. These results indicate that analytical kits developed for human clinical chemistry are not always useful for veterinary purposes and that precautions should be taken when nonhuman samples are analyzed. © 1997 Elsevier Science B.V.
- Enzymatic analysis
- Fatty acids