Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

E. Pileri, M. Cortey, F. Rodríguez, M. Sibila, L. Fraile, J. Segalés

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6 Citations (Scopus)

Abstract

© 2014 Elsevier Ltd. The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n=1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r2≥0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.
Original languageEnglish
Pages (from-to)429-432
JournalVeterinary Journal
Volume201
Issue number3
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • ELISA
  • Immunoperoxidase monolayer assay
  • Porcine circovirus type 2
  • Serology

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