Comparison of the f-HPV typing™ and Hybrid Capture II® assays for detection of high-risk HPV genotypes in cervical samples

María Paz Cañadas, Vincenzo Cirigliano, Laila Darwich, Guillermo Sirera, Josep Coll, Bonaventura Clotet, Sebastian Videla

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Human papillomavirus genotyping is being considered in cervical screening programs and for monitoring the effectiveness of HPV vaccination. Both approaches require access to fast, easy and high-throughput technology. The aim of this study was to compare a new commercial assay (f-HPV typing™) with the Hybrid Capture II® (HC2) to detect HPV infection. The F-HPV typing is a multiplex fluorescent PCR method recognizing E6 and E7 regions of 13 high-risk (HR) HPV types, the same set of HR-types targeted HC2 test. A subset of 157 cervical samples was tested with both assays. The percentage of positive HR-HPV DNA samples was 24% (37/155) by HC2 and 33% (49/155) by f-HPV typing. Concordant results were found in 133/155 (overall agreement, 85.8%; Cohen's kappa = 0.65). The analytical sensitivity and specificity of f-HPV were 97.6 and 93, respectively. In conclusion, this study shows that the f-HPV assay provides a good alternative to HC2 to detect HPV infection, allowing simple and rapid HPV genotyping and detecting multiple infections. © 2012 Elsevier B.V..
Original languageEnglish
Pages (from-to)14-18
JournalJournal of Virological Methods
Volume183
Issue number1
DOIs
Publication statusPublished - 1 Jul 2012

Keywords

  • HPV
  • HPV genotyping
  • Hybrid capture-2
  • Linear Array
  • Multiplex-PCR
  • Test comparison

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