Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R 2) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs. © 2010 Elsevier Ltd.
|Publication status||Published - 1 Oct 2011|
- Hepatitis E virus
- Muscle fluid