Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs

Maribel Casas, Sonia Pina, Bibiana Peralta, Enric Mateu, Jordi Casal, Marga Martín

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R 2) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs. © 2010 Elsevier Ltd.
Original languageEnglish
Pages (from-to)179-180
JournalVeterinary Journal
Volume190
DOIs
Publication statusPublished - 1 Oct 2011

Keywords

  • ELISA
  • Hepatitis E virus
  • Muscle fluid
  • Pigs
  • Serology

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