Comparison of 2 molecular assays and a serologic test in diagnosing Mycoplasma pneumoniae infection in paediatrics patients

Blanco Silvia, Fuenzalida Loreto, Bas Albert, Prat Cristina, Ramírez Aida, Matas Lurdes, Rodrigo Carlos, Ausina Vicente

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Two commercial polymerase chain reaction (PCR) assays (a real-time PCR [Cepheid] and an oligochromatographic test [Speed-oligo]) and 1 serology test (Serodia-Myco II) for detecting Mycoplasma pneumoniae in nasopharyngeal aspirates and serum samples were studied. Among the 145 samples, 32 serum pairs were serologically positive for M. pneumoniae. Of these, in 30 nasopharyngeal aspirates, M. pneumoniae was detected using the real-time PCR assay and 25 using Speed-oligo, corresponding to a sensitivity of 93.7% and 78.1%, respectively. Among the 94 samples with negative serology, we only obtained 1 positive result by real-time PCR assay. In the group of samples from healthy children, no positive results were obtained. © 2011 Elsevier Inc.
Original languageEnglish
Pages (from-to)463-466
JournalDiagnostic Microbiology and Infectious Disease
Volume71
Issue number4
DOIs
Publication statusPublished - 1 Dec 2011

Keywords

  • M. pneumoniae
  • PCR
  • Serology

Fingerprint Dive into the research topics of 'Comparison of 2 molecular assays and a serologic test in diagnosing Mycoplasma pneumoniae infection in paediatrics patients'. Together they form a unique fingerprint.

Cite this