Comparison between conventional culture and NAATs for the microbiological diagnosis in gonococcal infection

J. Serra-Pladevall, E. Caballero, G. Roig, R. Juvé, M. J. Barbera, A. Andreu

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8 Citations (Scopus)

Abstract

© 2015 Elsevier Inc. Gonorrhea is a public health problem. Fast diagnostics is necessary. The aim of this study was to compare the diagnostic yield of culture with that of polymerase chain reaction (PCR) in gonococcal infection. This is a study comparing the results of Neisseria gonorrhoeae detection by culture versus PCR from July to December 2012. Molecular diagnosis was performed by real-time PCR using the Versant CT/GC DNA 1.0 assay. In the 768 specimens, 96.9% the results were concordant. In 3.1%, the results were discordant, being PCR-positive and culture-negative in 21 cases and PCR-negative and culture-positive in 3. The sensitivity, specificity, positive predictive value, and negative predictive value for culture were 86.2%, 99.8%, 99.2%, and 96.7%, and for PCR, 98.7%, 100%, 100% and 99.7%, respectively. In laboratories where antimicrobial susceptibility is monitored, an effective approach would be to perform culture in addition to PCR in symptomatic patients.
Original languageEnglish
Pages (from-to)341-343
JournalDiagnostic Microbiology and Infectious Disease
Volume83
Issue number4
DOIs
Publication statusPublished - 1 Dec 2015

Keywords

  • Culture
  • Diagnostics
  • Gonorrhea
  • Neisseria gonorrhoeae
  • PCR

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