Comparative study of electron mediators used in the electrochemical oxidation of NADH

Beatriu Prieto-Simón; Esteve Fàbregas

doi:10.1016/j.bios.2003.10.010

Comparative study of electron mediators used in the electrochemical oxidation of NADH

Beatriu Prieto-Simón, Esteve Fàbregas

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

92 Citations (Scopus)

Abstract

A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared. © 2003 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	1131-1138
Journal	Biosensors and Bioelectronics
Volume	19
Issue number	10
DOIs	https://doi.org/10.1016/j.bios.2003.10.010
Publication status	Published - 15 May 2004

Keywords

Amperometric detection of NADH
Chemically modified electrodes
Redox mediators

Access to Document

10.1016/j.bios.2003.10.010

Cite this

@article{9a50421c36cc4da3973a216263370510,

title = "Comparative study of electron mediators used in the electrochemical oxidation of NADH",

abstract = "A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared. {\textcopyright} 2003 Elsevier B.V. All rights reserved.",

keywords = "Amperometric detection of NADH, Chemically modified electrodes, Redox mediators",

author = "Beatriu Prieto-Sim{\'o}n and Esteve F{\`a}bregas",

year = "2004",

month = may,

day = "15",

doi = "10.1016/j.bios.2003.10.010",

language = "English",

volume = "19",

pages = "1131--1138",

number = "10",

}

TY - JOUR

T1 - Comparative study of electron mediators used in the electrochemical oxidation of NADH

AU - Prieto-Simón, Beatriu

AU - Fàbregas, Esteve

PY - 2004/5/15

Y1 - 2004/5/15

N2 - A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared. © 2003 Elsevier B.V. All rights reserved.

AB - A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared. © 2003 Elsevier B.V. All rights reserved.

KW - Amperometric detection of NADH

KW - Chemically modified electrodes

KW - Redox mediators

U2 - 10.1016/j.bios.2003.10.010

DO - 10.1016/j.bios.2003.10.010

M3 - Article

SN - 0956-5663

VL - 19

SP - 1131

EP - 1138

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 10

ER -

Comparative study of electron mediators used in the electrochemical oxidation of NADH

Abstract

Keywords

Access to Document

Fingerprint

Cite this