Comparative study of different fluorescent dyes for the detection of proteins on membranes using the peroxyoxalate chemiluminescent reaction

Doris Salerno, Joan Ramon Daban

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

We have previously shown that the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H2O2 chemiluminescent reaction in acetone can be used for the detection of proteins labeled with the fluorescent reagent 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) on polyvinylidene difluoride (PVDF) membranes. To improve this method, in this work we have designed and constructed a cell that allows us to perform this chemiluminescent reaction on PVDF membranes with a homogeneous distribution of the reagents. Using this cell we have examined the analytical properties of several recently developed fluorescent protein dyes chemically different from MDPF. We have found that the metal chelate dye SYPRO Ruby can also be excited by the high-energy intermediate produced in the TCPO-H2O2 reaction. © 2003 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)75-81
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume793
Issue number1
DOIs
Publication statusPublished - 5 Aug 2003

Keywords

  • Fluorescent dyes
  • Peroxyoxalate chemiluminescence
  • Proteins

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