Comparative Genomic Hybridization Analysis Reveals New Different Subgroups in Early-stage Bladder Tumors

Esther Prat, Javier del Rey, Immaculada Ponsa, Marga Nadal, Jordi Camps, Alberto Plaja, Mercedes Campillo, Ferran Algaba, Antoni Gelabert, Rosa Miró

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Objectives: To classify bladder tumors according to their genomic imbalances and evaluate their association with patient's outcome. Methods: Sixty-three superficially and minimally invasive bladder tumors were analyzed by conventional comparative genomic hybridization. Subtelomeric screening in 15 of these tumors was performed by multiplex ligation-dependent probe amplification. Results: Losses of 9q and 9p (32% and 25% of all cases, respectively) as well as gains of chromosomes Xq and Xp (28% and 25%, respectively) were the most frequent chromosome imbalances. Losses of 8p and gains in 1q and 8q were detected in >20% of cases. Tumors were classified into 3 groups according to their individualized pattern of gains and losses. The largest group was characterized by few chromosome imbalances, presenting 77% and 49% of the Ta and T1 tumors, respectively. Another group characterized by chromosomal gains, was composed of equal number of Ta and T1 tumors, with +1q and +17q gains being the most common imbalances. A minority group was characterized by chromosomal losses on 11q, 5q, and 6q. The multiplex ligation-dependent probe amplification study showed good correlation with comparative genomic hybridization results. With regard to the biological significance of this classification, a remarkable fact is that this minority group composed mainly of T1 tumors, showed a significant decrease in patient overall survival. Conclusions: Our data suggest that superficial carcinomas of the bladder can be subdivided into a larger number of subclasses than had previously been expected. Our results also demonstrate a decreased survival among patients whose tumors show more genomic losses than gains. Crown Copyright © 2010.
Original languageEnglish
Pages (from-to)347-355
JournalUrology
Volume75
Issue number2
DOIs
Publication statusPublished - 1 Feb 2010

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