TY - JOUR
T1 - Combining magnetic nanoparticles and icosahedral boron clusters in biocompatible inorganic nanohybrids for cancer therapy
AU - Oleshkevich, Elena
AU - Morancho, Anna
AU - Saha, Arpita
AU - Galenkamp, Koen M.O.
AU - Grayston, Alba
AU - Crich, Simonetta Geninatti
AU - Alberti, Diego
AU - Protti, Nicoletta
AU - Comella, Joan X.
AU - Teixidor, Francesc
AU - Rosell, Anna
AU - Viñas, Clara
PY - 2019/8/1
Y1 - 2019/8/1
N2 - © 2019 Elsevier Inc. The potential biomedical applications of the MNPs nanohybrids coated with m-carboranylphosphinate (1-MNPs) as a theranostic biomaterial for cancer therapy were tested. The cellular uptake and toxicity profile of 1-MNPs from culture media by human brain endothelial cells (hCMEC/D3) and glioblastoma multiform A172 cell line were demonstrated. Prior to testing 1-MNPs' in vitro toxicity, studies of colloidal stability of the 1-MNPs' suspension in different culture media and temperatures were carried out. TEM images and chemical titration confirmed that 1-MNPs penetrate into cells. Additionally, to explore 1-MNPs' potential use in Boron Neutron Capture Therapy (BNCT) for treating cancer locally, the presence of the m-carboranyl coordinated with the MNPs core after uptake was proven by XPS and EELS. Importantly, thermal neutrons irradiation in BNCT reduced by 2.5 the number of cultured glioblastoma cells after 1-MNP treatment, and the systemic administration of 1-MNPs in mice was well tolerated with no major signs of toxicity.
AB - © 2019 Elsevier Inc. The potential biomedical applications of the MNPs nanohybrids coated with m-carboranylphosphinate (1-MNPs) as a theranostic biomaterial for cancer therapy were tested. The cellular uptake and toxicity profile of 1-MNPs from culture media by human brain endothelial cells (hCMEC/D3) and glioblastoma multiform A172 cell line were demonstrated. Prior to testing 1-MNPs' in vitro toxicity, studies of colloidal stability of the 1-MNPs' suspension in different culture media and temperatures were carried out. TEM images and chemical titration confirmed that 1-MNPs penetrate into cells. Additionally, to explore 1-MNPs' potential use in Boron Neutron Capture Therapy (BNCT) for treating cancer locally, the presence of the m-carboranyl coordinated with the MNPs core after uptake was proven by XPS and EELS. Importantly, thermal neutrons irradiation in BNCT reduced by 2.5 the number of cultured glioblastoma cells after 1-MNP treatment, and the systemic administration of 1-MNPs in mice was well tolerated with no major signs of toxicity.
KW - Boron neutron capture therapy
KW - Iron oxide nanoparticles
KW - m-Carboranyl
KW - Nanomedicine
KW - Phosphinate
U2 - 10.1016/j.nano.2019.03.008
DO - 10.1016/j.nano.2019.03.008
M3 - Article
C2 - 31059794
VL - 20
M1 - 101986
ER -
