Combined effect of inorganic carbon limitation and inhibition by free ammonia and free nitrous acid on ammonia oxidizing bacteria

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Abstract

The inhibitory effect of free ammonia (NH3 or FA) and free nitrous acid (HNO2 or FNA) on the ammonia oxidizing bacteria (AOB) and the dependence of the AOB activity on the concentration of total inorganic carbon (TIC) are well-established. In contrast, less is know about the effect of high FA and FNA concentrations in combination with TIC limitation. Respirometric tests performed with an enriched AOB sludge (81% of AOB as measured with fluorescent in situ hybridization) established that AOB inhibition by FA under TIC limitation was higher than under non-limiting TIC conditions (Haldane inhibition coefficients of 139 and 376 mg NH3 L-1). AOB affinity for FA decreased under TIC limitation conditions (half-saturation coefficient of 0.28 mg NH3 L-1 without TIC limitation and 4.3 mg NH3 L-1 with TIC limitation). Higher inhibition by FNA was observed when TIC was limited since the non-competitive inhibition coefficient decreased from 1.31 mg HNO2 L-1 (without TIC limitation) to 0.21 mg HNO2 L-1 (with TIC limitation). This study demonstrates that AOB inhibitions by FA and FNA are amplified with TIC limitation and consequently, AOB dynamics are strongly modified under TIC limitation conditions. © 2010 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)6051-6058
JournalBioresource Technology
Volume101
Issue number15
DOIs
Publication statusPublished - 1 Aug 2010

Keywords

  • FISH
  • Kinetic modeling
  • Oxygen uptake rate
  • Partial nitrification
  • Starvation

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