Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin

Alex Perálvarez-Marín, Francesc Sepulcre, Mercedes Márquez, Maria Grazia Proietti, Esteve Padrós

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1 Citation (Scopus)

Abstract

We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn 2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn 2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell. © 2011 European Biophysical Societies' Association.
Original languageEnglish
Pages (from-to)1007-1012
JournalEuropean Biophysics Journal
Volume40
Issue number8
DOIs
Publication statusPublished - 1 Aug 2011

Keywords

  • Bacteriorhodopsin
  • Cation binding
  • EXAFS
  • Lipidic cubic phase
  • Membrane protein

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