Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin

Alex Perálvarez-Marín; Francesc Sepulcre; Mercedes Márquez; Maria Grazia Proietti; Esteve Padrós

doi:10.1007/s00249-011-0714-3

Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin

Alex Perálvarez-Marín, Francesc Sepulcre, Mercedes Márquez, Maria Grazia Proietti, Esteve Padrós

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn 2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn 2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell. © 2011 European Biophysical Societies' Association.

Original language	English
Pages (from-to)	1007-1012
Journal	European Biophysics Journal
Volume	40
Issue number	8
DOIs	https://doi.org/10.1007/s00249-011-0714-3
Publication status	Published - 1 Aug 2011

Keywords

Bacteriorhodopsin
Cation binding
EXAFS
Lipidic cubic phase
Membrane protein

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title = "Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin",

abstract = "We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn 2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn 2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell. {\textcopyright} 2011 European Biophysical Societies' Association.",

keywords = "Bacteriorhodopsin, Cation binding, EXAFS, Lipidic cubic phase, Membrane protein",

author = "Alex Per{\'a}lvarez-Mar{\'i}n and Francesc Sepulcre and Mercedes M{\'a}rquez and Proietti, {Maria Grazia} and Esteve Padr{\'o}s",

year = "2011",

month = aug,

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doi = "10.1007/s00249-011-0714-3",

language = "English",

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Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin. / Perálvarez-Marín, Alex; Sepulcre, Francesc; Márquez, Mercedes; Proietti, Maria Grazia; Padrós, Esteve.

In: European Biophysics Journal, Vol. 40, No. 8, 01.08.2011, p. 1007-1012.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin

AU - Perálvarez-Marín, Alex

AU - Sepulcre, Francesc

AU - Márquez, Mercedes

AU - Proietti, Maria Grazia

AU - Padrós, Esteve

PY - 2011/8/1

Y1 - 2011/8/1

N2 - We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn 2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn 2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell. © 2011 European Biophysical Societies' Association.

AB - We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn 2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn 2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell. © 2011 European Biophysical Societies' Association.

KW - Bacteriorhodopsin

KW - Cation binding

KW - EXAFS

KW - Lipidic cubic phase

KW - Membrane protein

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DO - 10.1007/s00249-011-0714-3

M3 - Article

VL - 40

SP - 1007

EP - 1012

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - 8

ER -