Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants

Anna Bigas; M. Elena Garrido; Ignacio Badiola; Jordi Barbé; Montserrat Llagostera

Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants

Anna Bigas, M. Elena Garrido, Ignacio Badiola, Jordi Barbé, Montserrat Llagostera

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

The bacterial thyA gene encodes the enzyme thymidylate synthase, which is essential for dTMP synthesis and, consequently, for DNA replication. In this work, a Haemophilus parasuis thyA mutant was constructed in order to analyze its colonization characteristics and its capacity to generate serum bactericidal activity in infected guinea pigs. The data showed that colonization by the H. parasuis thyA mutant was much less than that of the wild-type strain. Nevertheless, the mutant generated a strong immunogenic response in the host, as detected by measuring serum bactericidal activity.

Original language	English
Pages (from-to)	297-301
Journal	International Microbiology
Volume	9
Issue number	4
Publication status	Published - 1 Jan 2006

Keywords

Bactericidal activity
Colonization capacity
Haemophilus parasuis
Immunogenic response
thy mutants
Vaccine strains

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title = "Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants",

abstract = "The bacterial thyA gene encodes the enzyme thymidylate synthase, which is essential for dTMP synthesis and, consequently, for DNA replication. In this work, a Haemophilus parasuis thyA mutant was constructed in order to analyze its colonization characteristics and its capacity to generate serum bactericidal activity in infected guinea pigs. The data showed that colonization by the H. parasuis thyA mutant was much less than that of the wild-type strain. Nevertheless, the mutant generated a strong immunogenic response in the host, as detected by measuring serum bactericidal activity.",

keywords = "Bactericidal activity, Colonization capacity, Haemophilus parasuis, Immunogenic response, thy mutants, Vaccine strains",

author = "Anna Bigas and Garrido, {M. Elena} and Ignacio Badiola and Jordi Barb{\'e} and Montserrat Llagostera",

year = "2006",

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volume = "9",

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T1 - Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants

AU - Bigas, Anna

AU - Garrido, M. Elena

AU - Badiola, Ignacio

AU - Barbé, Jordi

AU - Llagostera, Montserrat

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The bacterial thyA gene encodes the enzyme thymidylate synthase, which is essential for dTMP synthesis and, consequently, for DNA replication. In this work, a Haemophilus parasuis thyA mutant was constructed in order to analyze its colonization characteristics and its capacity to generate serum bactericidal activity in infected guinea pigs. The data showed that colonization by the H. parasuis thyA mutant was much less than that of the wild-type strain. Nevertheless, the mutant generated a strong immunogenic response in the host, as detected by measuring serum bactericidal activity.

AB - The bacterial thyA gene encodes the enzyme thymidylate synthase, which is essential for dTMP synthesis and, consequently, for DNA replication. In this work, a Haemophilus parasuis thyA mutant was constructed in order to analyze its colonization characteristics and its capacity to generate serum bactericidal activity in infected guinea pigs. The data showed that colonization by the H. parasuis thyA mutant was much less than that of the wild-type strain. Nevertheless, the mutant generated a strong immunogenic response in the host, as detected by measuring serum bactericidal activity.

KW - Bactericidal activity

KW - Colonization capacity

KW - Haemophilus parasuis

KW - Immunogenic response

KW - thy mutants

KW - Vaccine strains

M3 - Article

VL - 9

SP - 297

EP - 301

JO - International Microbiology

JF - International Microbiology

SN - 1139-6709

IS - 4

ER -