Colonic gas homeostasis: Mechanisms of adaptation following HOST-G904 galactooligosaccharide use in humans

M. Mego, A. Accarino, G. Tzortzis, J. Vulevic, G. Gibson, F. Guarner, F. Azpiroz

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19 Citations (Scopus)


© 2017 John Wiley & Sons Ltd Background: We have shown that a galactooligosaccharide prebiotic administration (HOST-G904) initially increased intestinal gas production and this increase declined back to baseline after 2 week administration. Our aim was to determine the mechanism of microbiota adaptation; i.e., to determine whether the net reduction is due to decreased overall production or increased gas consumption. Methods: In 10 healthy subjects, intestinal gas production and intraluminal disposal was measured before, at the beginning and after 2 week of HOST-G904 prebiotic administration. Anal gas was collected for 4 hour after a probe meal. Paired studies were performed without and with high-rate infusion of exogenous gas (24 mL/min) into the jejunum to wash-out the endogenous gas produced by bacterial fermentation. The exogenous gas infused was labeled (5% SF6) to calculate the proportion of endogenous gas evacuated. Key Results: The volume of intestinal gas produced i.e., endogenous gas washed-out, increased by 37% at the beginning of HOST-G904 administration (P=.049 vs preadministration) and decreased down to preadministration level after 2 week administration (P=.030 vs early administration). The proportion of gas eliminated from the lumen before reaching the anus tended to increase after 2-week administration (87±3% vs 78±5% preadministration; P=.098). Conclusions & Inferences: Adaptation to regular consumption of HOST-G904 prebiotic involves a shift in microbiota metabolism toward low-gas producing pathways, with a non-significant increase in gas-consuming activity. Hence, regular consumption of HOST-G904 regulates intestinal gas metabolism: less gas is produced and a somewhat larger proportion of it is consumed.
Original languageEnglish
Article numbere13080
JournalNeurogastroenterology and Motility
Issue number9
Publication statusPublished - 1 Sept 2017


  • diet
  • fiber
  • gut microbiota
  • intestinal gas
  • prebiotics


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