Cloning of the Rhodobacter sphaeroides hisI gene: Unifunctionality of the encoded protein and lack of linkage to other his genes

Esther Oriol, Sebastián Méndez-Álvarez, Jordi Barbé, Isidre Gibert

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5 Citations (Scopus)

Abstract

The Rhodobacter sphaeroides 2.4.1 hisI gene, which encodes a phosphoribosyl-AMP-cyclohydrolase that catalyses the third step in the histidine biosynthetic pathway, has been isolated from a genomic library of this phototrophic bacterium by complementation of an Escherichia coli hisI mutant. Analysis of the nucleotide sequence of the R. sphaeroides hisI gene reveals that it encodes a deduced product of 119 aa with a predicted molecular mass of 13.4 kDa. In contrast to the situation in E. coli, the R. sphaeroides hisI gene encodes a unifunctional protein and it is not linked to the hisE gene. The absence of a single histidine operon like that of E. coli was confirmed by PFGE experiments and complementation analysis of a R. sphaeroides hisI mutant that was constructed by marker exchange. The location of hisI in the R. sphaeroides genome has been determined to be at map co-ordinate 2275 ± 20 of chromosome I.
Original languageEnglish
Pages (from-to)2071-2078
JournalMicrobiology
Volume142
Issue number8
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • Chromosome mapping
  • hisI gene
  • Histidine biosynthesis
  • Rhodobacter sphaeroides

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