Cloning and characterization of the recA gene of Paracoccus denitrificans and construction of a recA-deficient mutant

Antonio R. Fernandez De Henestrosa, Alfonso Del Rey, Raül Tarragó, Jordi Barbé

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4 Citations (Scopus)

Abstract

The recA gene of Paracoccus denitrificans has been isolated from a genomic library by hybridization with the Rhodobacter sphaeroides recA gene. Its complete nucleotide sequence consists of 1071 bp encoding a polypeptide of 356 amino acids. Nucleotide sequence analysis of the P. denitrificans recA gene revealed the closest identities with the R. sphaeroides and the Rhodobacter capsulatus recA genes. Nevertheless, and surprisingly, recA genes of these two phototrophic bacteria are not DNA damage-inducible when introduced into P. denitrificans cells, whereas recA genes of both P. denitrificans and Rhizobium etli are. These results suggest that the promoters of P. denitrificans and R. etli recA genes have a similar regulatory sequence. A recA-defective mutant of P. denitrificans has also been constructed by replacement of the active recA gene by an in vitro inactivated gene copy.
Original languageEnglish
Pages (from-to)209-213
JournalFEMS Microbiology Letters
Volume147
Issue number2
DOIs
Publication statusPublished - 15 Feb 1997

Keywords

  • Paracoccus denitrificans
  • recA gene
  • recA mutant
  • Rhizobium etli
  • Rhodobacter sphaeroides

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