Circular dichroism spectra of cyclodextrins-ketoprofen inclusion complexes Determination of enantiomeric purity

Marcelo Blanco, Jordi Coello, Hortensia Iturriaga, Santiago Maspoch, Carlos Pérez-Maseda

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)

Abstract

The formation of inclusion complexes among various cyclodextrins (viz. α, β, γ, trimethyl-β- and hydroxypropyl-β-cyclodextrin) and the S(+) and R(-) enantiomers of ketoprofen was studied by using the circular dichroism (CD) technique. All the cyclodextrins studied, except α-cyclodextrin, introduce marked differences between the CD spectra for the S(+) and R(-) enantiomer. A new method for determining the enantiomeric purity in mixtures of both ketoprofen enantiomers based on the use of β-cyclodextrin as chiral selector and partial least-squares regression (PLSR) for calibration is proposed. The method provides relative standard errors (RSE) of calibration and prediction of the order of 2%. It was also applied to the determination of the enantiomeric excess of eutomer S(+) in the presence of distomer R(-) contents below 5%. The good results provided by the proposed method and its analytical expeditiousness make it a competitive alternative to chromatographic methods for the same purpose. Copyright (C) 2000 Elsevier Science B.V.
Original languageEnglish
Pages (from-to)233-245
JournalAnalytica Chimica Acta
Volume407
Issue number1-2
DOIs
Publication statusPublished - 29 Feb 2000

Keywords

  • Circular dichroism spectroscopy
  • Cyclodextrins
  • Enantiomers
  • Ketoprofen
  • Multivariate calibration
  • Pharmaceutical analysis

Fingerprint

Dive into the research topics of 'Circular dichroism spectra of cyclodextrins-ketoprofen inclusion complexes Determination of enantiomeric purity'. Together they form a unique fingerprint.

Cite this