Chromosome map of the phototrophic anoxygenic bacterium Chromatium vinosum

N. Gaju; V. Pavón; I. Marin; I. Esteve; R. Guerrero; R. Amils

doi:10.1016/0378-1097(95)00016-X

Chromosome map of the phototrophic anoxygenic bacterium Chromatium vinosum

N. Gaju, V. Pavón, I. Marin, I. Esteve, R. Guerrero, R. Amils

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

The genome of Chromatium vinosum has been characterized using pulsed field gel electrophoresis. Two restriction endonucleases, AseI and SpeI, generated DNA fragments of size distributions suitable for mapping the genome of the anoxyphotobacterium C. vinosum DSM 180. AseI produced 24 fragments ranging from 367 to 10.8 kb and SpeI yielded 13 fragments from 720 to 12 kb. A total genome size of 3.674 Mb was determined by summing the fragment lengths in each of the digests generated using the different restriction endonucleases. Intact total DNA from C. vinosum shows the presence of three extrachromosomal elements. Three rRNA regions were located in the strain DSM 180. Restriction patterns of the strain DSM 180 have been compared with ATCC 17899 and DSM 185 of the same species. © 1995.

Original language	English
Pages (from-to)	241-247
Journal	FEMS Microbiology Letters
Volume	126
Issue number	3
DOIs	https://doi.org/10.1016/0378-1097(95)00016-X
Publication status	Published - 1 Mar 1995

Keywords

Chromatium vinosum
Chromosome map
Pulsed Field Gel Electrophoresis
rRNA regions

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title = "Chromosome map of the phototrophic anoxygenic bacterium Chromatium vinosum",

abstract = "The genome of Chromatium vinosum has been characterized using pulsed field gel electrophoresis. Two restriction endonucleases, AseI and SpeI, generated DNA fragments of size distributions suitable for mapping the genome of the anoxyphotobacterium C. vinosum DSM 180. AseI produced 24 fragments ranging from 367 to 10.8 kb and SpeI yielded 13 fragments from 720 to 12 kb. A total genome size of 3.674 Mb was determined by summing the fragment lengths in each of the digests generated using the different restriction endonucleases. Intact total DNA from C. vinosum shows the presence of three extrachromosomal elements. Three rRNA regions were located in the strain DSM 180. Restriction patterns of the strain DSM 180 have been compared with ATCC 17899 and DSM 185 of the same species. {\textcopyright} 1995.",

keywords = "Chromatium vinosum, Chromosome map, Pulsed Field Gel Electrophoresis, rRNA regions",

author = "N. Gaju and V. Pav{\'o}n and I. Marin and I. Esteve and R. Guerrero and R. Amils",

year = "1995",

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doi = "10.1016/0378-1097(95)00016-X",

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TY - JOUR

T1 - Chromosome map of the phototrophic anoxygenic bacterium Chromatium vinosum

AU - Gaju, N.

AU - Pavón, V.

AU - Marin, I.

AU - Esteve, I.

AU - Guerrero, R.

AU - Amils, R.

PY - 1995/3/1

Y1 - 1995/3/1

N2 - The genome of Chromatium vinosum has been characterized using pulsed field gel electrophoresis. Two restriction endonucleases, AseI and SpeI, generated DNA fragments of size distributions suitable for mapping the genome of the anoxyphotobacterium C. vinosum DSM 180. AseI produced 24 fragments ranging from 367 to 10.8 kb and SpeI yielded 13 fragments from 720 to 12 kb. A total genome size of 3.674 Mb was determined by summing the fragment lengths in each of the digests generated using the different restriction endonucleases. Intact total DNA from C. vinosum shows the presence of three extrachromosomal elements. Three rRNA regions were located in the strain DSM 180. Restriction patterns of the strain DSM 180 have been compared with ATCC 17899 and DSM 185 of the same species. © 1995.

AB - The genome of Chromatium vinosum has been characterized using pulsed field gel electrophoresis. Two restriction endonucleases, AseI and SpeI, generated DNA fragments of size distributions suitable for mapping the genome of the anoxyphotobacterium C. vinosum DSM 180. AseI produced 24 fragments ranging from 367 to 10.8 kb and SpeI yielded 13 fragments from 720 to 12 kb. A total genome size of 3.674 Mb was determined by summing the fragment lengths in each of the digests generated using the different restriction endonucleases. Intact total DNA from C. vinosum shows the presence of three extrachromosomal elements. Three rRNA regions were located in the strain DSM 180. Restriction patterns of the strain DSM 180 have been compared with ATCC 17899 and DSM 185 of the same species. © 1995.

KW - Chromatium vinosum

KW - Chromosome map

KW - Pulsed Field Gel Electrophoresis

KW - rRNA regions

U2 - 10.1016/0378-1097(95)00016-X

DO - 10.1016/0378-1097(95)00016-X

M3 - Article

VL - 126

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EP - 247

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