Chromosome map of the phototrophic anoxygenic bacterium Chromatium vinosum

N. Gaju, V. Pavón, I. Marin, I. Esteve, R. Guerrero, R. Amils

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

The genome of Chromatium vinosum has been characterized using pulsed field gel electrophoresis. Two restriction endonucleases, AseI and SpeI, generated DNA fragments of size distributions suitable for mapping the genome of the anoxyphotobacterium C. vinosum DSM 180. AseI produced 24 fragments ranging from 367 to 10.8 kb and SpeI yielded 13 fragments from 720 to 12 kb. A total genome size of 3.674 Mb was determined by summing the fragment lengths in each of the digests generated using the different restriction endonucleases. Intact total DNA from C. vinosum shows the presence of three extrachromosomal elements. Three rRNA regions were located in the strain DSM 180. Restriction patterns of the strain DSM 180 have been compared with ATCC 17899 and DSM 185 of the same species. © 1995.
Original languageEnglish
Pages (from-to)241-247
JournalFEMS Microbiology Letters
Volume126
Issue number3
DOIs
Publication statusPublished - 1 Mar 1995

Keywords

  • Chromatium vinosum
  • Chromosome map
  • Pulsed Field Gel Electrophoresis
  • rRNA regions

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