Chromosomal analysis of blastocyst derived from monopronucleated ICSI zygotes: Approach by double trophectoderm biopsy

Silvia Mateo, Francesca Vidal, Lluc Coll, Anna Veiga, Montserrat Boada

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

© 2017, Sociedade Brasileira de Reproducao Assistida. All rights reserved. Objective: This study aims to increase the knowledge about monopronucleated ICSI-derived blastocysts, analyzing trophectoderm biopsies by aCGH and FISH to evaluate their chromosome constitution. Methods: Fifteen monopronucleated ICSI-derived blastocysts were studied. Double trophectoderm biopsy was performed and analyzed by FISH and aCGH. The blastocysts were classified according to chromosome constitution. Disagreements between the two techniques were assessed. Results: Results obtained after FISH and aCGH analyses showed the following: 20% (3/15) and 60% (9/15) diploid females, respectively; 26.7% (4/15) and 26.7% (4/15) diploid males, respectively; and 53.3% (8/15) and 13.3% (2/15) mosaics, respectively. No mosaic male embryos were found using FISH or aCGH. There were disagreements in 40% (6/15) of the cases due to the higher detection of mosaicism by FISH compared to aCGH. Conclusions: The combination of FISH and aCGH has been shown to be a suitable approach to increase the knowledge about monopronucleated ICSI-derived embryos. FISH analysis of blastocysts derived from monopronucleated ICSI zygotes enabled us to conclude that aCGH underestimates haploidy. Some diploid embryos diagnosed by aCGH are in fact mosaic. In cases where these embryos would be used for reproductive purposes, extra analysis of parental genome origin is recommended.
Original languageEnglish
Pages (from-to)203-207
JournalJornal Brasileiro de Reproducao Assistida
Volume21
Issue number3
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • ACGH
  • FISH
  • Monopronucleated zygote
  • Trophectoderm biopsy

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