TY - JOUR
T1 - Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria
AU - Barbe, J.
AU - Fernandez De Henestrosa, A. R.
AU - Calero, S.
AU - Gibert, I.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.
AB - We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.
U2 - 10.1128/jb.173.1.404-406.1991
DO - 10.1128/jb.173.1.404-406.1991
M3 - Article
VL - 173
SP - 404
EP - 406
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 1
ER -