Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria

J. Barbe, A. R. Fernandez De Henestrosa, S. Calero, I. Gibert

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.
Original languageEnglish
Pages (from-to)404-406
JournalJournal of Bacteriology
Volume173
Issue number1
DOIs
Publication statusPublished - 1 Jan 1991

Fingerprint Dive into the research topics of 'Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria'. Together they form a unique fingerprint.

Cite this