Abstract
We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.
Original language | English |
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Pages (from-to) | 404-406 |
Journal | Journal of Bacteriology |
Volume | 173 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Jan 1991 |