Chlorogenic acid improves the quality of boar semen subjected to cooled storage at 15°C

B. A. Pereira, B. R. Chaves, M. C. Teles, T. P. Pontelo, C. R. Oliveira, R. V. de Souza, J. E. Rodríguez-Gil, M. G. Zangeronimo

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11 Citations (Scopus)


© 2018 Blackwell Verlag GmbH The aim of this study was to investigate the effect of chlorogenic acid (ChA) in boar semen stored at 15°C. Twelve ejaculates were processed into insemination doses at different concentrations of ChA (0.0, 1.5, 3.0, 4.0 and 6.0 mg/ml) or vitamin E (200 μl/ml) as positive control. Semen was analysed after 0, 24 and 72 hr of storage. ChA improved (p <.05) sperm motility, acrosome integrity and mitochondrial activity in all periods of storage. Furthermore, after 24 hr of storage, ChA above 1.5 mg/ml supported the sperm viability until 120 min after reheating (p <.05). Both ChA and vitamin E were similarly efficient in increasing the antioxidant capacity of semen, reducing the malondialdehyde levels before and after 72 hr of storage (p <.05). However, with 72 hr of storage, ChA at 3.0 mg/ml improved the mitochondrial activity over vitamin E (p <.05). In conclusion, results suggest that the concentration of 3.2 mg/ml of ChA is the best for semen stored for up to 24 hr. However, for semen stored for a longer period, 6.0 mg/ml or more should be used.
Original languageEnglish
Article numbere12978
Issue number5
Publication statusPublished - 1 Jun 2018


  • antioxidant
  • boar spermatozoa
  • chlorogenic acid
  • oxidative damage
  • semen storage


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