Chimeric calicivirus-like particles elicit protective anti-viral cytotoxic responses without adjuvant

E. Crisci, H. Almanza, I. Mena, L. Córdoba, E. Gómez-Casado, J. R. Castón, L. Fraile, J. Bárcena, M. Montoya

    Research output: Contribution to journalArticleResearchpeer-review

    21 Citations (Scopus)

    Abstract

    We have analyzed the potential of virus-like particles (VLPs) from rabbit hemorrhagic disease virus (RHDV) as a delivery system for foreign T cell epitopes. To accomplish this goal, we generated chimeric RHDV-VLPs incorporating a CD8+ T cell epitope (SIINFEKL) derived from chicken ovalbumin (OVA). The OVA epitope was inserted in the capsid protein (VP60) of RHDV at two different locations: 1) the N-terminus, predicted to be facing to the inner core of the VLPs, and 2) a novel insertion site predicted to be located within an exposed loop. Both constructions correctly assembled into VLPs. In vitro, the chimeric VLPs activated dendritic cells for TNF-α secretion and they were processed and presented to specific T cells. In vivo, mice immunized with the chimeric VLPs without adjuvant were able to induce specific cellular responses mediated by cytotoxic and memory T cells. More importantly, immunization with chimeric VLPs was able to resolve an infection by a recombinant vaccinia virus expressing OVA protein. © 2009 Elsevier Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)303-312
    JournalVirology
    Volume387
    Issue number2
    DOIs
    Publication statusPublished - 10 May 2009

    Keywords

    • Dendritic cells
    • RHDV virus-like particles
    • Vaccine vector
    • Vaccinia virus infection

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