Characterization of the hepatic insulin receptor undergoing internalization through clathrin-coated vesicles and endosomes

Marta Jose; Josep A. Biosca; Ramon Trujillo; Emilio Itarte

doi:10.1016/0014-5793(93)80696-R

Characterization of the hepatic insulin receptor undergoing internalization through clathrin-coated vesicles and endosomes

Marta Jose, Josep A. Biosca, Ramon Trujillo, Emilio Itarte

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

Administration of insulin to rats caused a transient increase in the amount of hepatic insulin receptor present in clathrin-coated vesicles and endosomes. However, the total 'in vitro' insulin stimulated tyrosine kinase activity of the receptor present in endosomes did not vary when expressed per mg of protein and decreased when expressed per β-subunit content. A decrease in the endogenous phospho tyrosine content of the receptor β-subunit was observed in endosomes in response to insulin. This indicates that a fraction of the internalized receptor is dephosphorylated in endosomes, which renders it unable to become stimulated by insulin 'in vitro'. © 1993.

Original language	English
Pages (from-to)	286-288
Journal	FEBS Letters
Volume	334
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)80696-R
Publication status	Published - 22 Nov 1993

Keywords

Clathrin-coated vesicle
Endosome
Insulin receptor
Internalization
Protein phosphorylation
Rat liver

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10.1016/0014-5793(93)80696-R

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title = "Characterization of the hepatic insulin receptor undergoing internalization through clathrin-coated vesicles and endosomes",

abstract = "Administration of insulin to rats caused a transient increase in the amount of hepatic insulin receptor present in clathrin-coated vesicles and endosomes. However, the total 'in vitro' insulin stimulated tyrosine kinase activity of the receptor present in endosomes did not vary when expressed per mg of protein and decreased when expressed per β-subunit content. A decrease in the endogenous phospho tyrosine content of the receptor β-subunit was observed in endosomes in response to insulin. This indicates that a fraction of the internalized receptor is dephosphorylated in endosomes, which renders it unable to become stimulated by insulin 'in vitro'. {\textcopyright} 1993.",

keywords = "Clathrin-coated vesicle, Endosome, Insulin receptor, Internalization, Protein phosphorylation, Rat liver",

author = "Marta Jose and Biosca, {Josep A.} and Ramon Trujillo and Emilio Itarte",

year = "1993",

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doi = "10.1016/0014-5793(93)80696-R",

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T1 - Characterization of the hepatic insulin receptor undergoing internalization through clathrin-coated vesicles and endosomes

AU - Jose, Marta

AU - Biosca, Josep A.

AU - Trujillo, Ramon

AU - Itarte, Emilio

PY - 1993/11/22

Y1 - 1993/11/22

N2 - Administration of insulin to rats caused a transient increase in the amount of hepatic insulin receptor present in clathrin-coated vesicles and endosomes. However, the total 'in vitro' insulin stimulated tyrosine kinase activity of the receptor present in endosomes did not vary when expressed per mg of protein and decreased when expressed per β-subunit content. A decrease in the endogenous phospho tyrosine content of the receptor β-subunit was observed in endosomes in response to insulin. This indicates that a fraction of the internalized receptor is dephosphorylated in endosomes, which renders it unable to become stimulated by insulin 'in vitro'. © 1993.

AB - Administration of insulin to rats caused a transient increase in the amount of hepatic insulin receptor present in clathrin-coated vesicles and endosomes. However, the total 'in vitro' insulin stimulated tyrosine kinase activity of the receptor present in endosomes did not vary when expressed per mg of protein and decreased when expressed per β-subunit content. A decrease in the endogenous phospho tyrosine content of the receptor β-subunit was observed in endosomes in response to insulin. This indicates that a fraction of the internalized receptor is dephosphorylated in endosomes, which renders it unable to become stimulated by insulin 'in vitro'. © 1993.

KW - Clathrin-coated vesicle

KW - Endosome

KW - Insulin receptor

KW - Internalization

KW - Protein phosphorylation

KW - Rat liver

U2 - 10.1016/0014-5793(93)80696-R

DO - 10.1016/0014-5793(93)80696-R

M3 - Article

VL - 334

SP - 286

EP - 288

IS - 3

ER -

Characterization of the hepatic insulin receptor undergoing internalization through clathrin-coated vesicles and endosomes

Abstract

Keywords

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