Characterization of the hepatic insulin receptor undergoing internalization through clathrin-coated vesicles and endosomes

Marta Jose, Josep A. Biosca, Ramon Trujillo, Emilio Itarte

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Administration of insulin to rats caused a transient increase in the amount of hepatic insulin receptor present in clathrin-coated vesicles and endosomes. However, the total 'in vitro' insulin stimulated tyrosine kinase activity of the receptor present in endosomes did not vary when expressed per mg of protein and decreased when expressed per β-subunit content. A decrease in the endogenous phospho tyrosine content of the receptor β-subunit was observed in endosomes in response to insulin. This indicates that a fraction of the internalized receptor is dephosphorylated in endosomes, which renders it unable to become stimulated by insulin 'in vitro'. © 1993.
Original languageEnglish
Pages (from-to)286-288
JournalFEBS Letters
Volume334
Issue number3
DOIs
Publication statusPublished - 22 Nov 1993

Keywords

  • Clathrin-coated vesicle
  • Endosome
  • Insulin receptor
  • Internalization
  • Protein phosphorylation
  • Rat liver

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