TY - JOUR
T1 - Characterization of the canine rostral ventricular-subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies
AU - Pumarola Batlle, Marti
AU - Arus Caralto, Carles
AU - Fondevila Palau, M. Dolores
AU - Fernandez Flores, Francisco
AU - García-Verdugo, José Manuel
AU - Martín-Ibáñez, Raquel
AU - Herranz, Cristina
AU - Canals, Josep María
N1 - Funding Information:
Ministerio de Economia y Competitividad, Grant Number: PLE2009-0089, SAF2012-37417; Spain, Grant Number: SAF2015-66505-R (to J.M.C.); Instituto de Salud Carlos III-Subdirección General de Evaluación, Grant Number: RD06/0010/0006; European Regional Development Fund (ERDF) CIBERNED (to J.A. and C.V.-A.); RETICS (Red de Terapia Celular), Grant Number: RD12/0019/0002; (to J.M.C.); Generalitat de Catalunya, Grant Number: 2014SGR-968; La Marató TV3, Grant Number: 20140130/1; Agència de Gestió d’Ajuts Universitaris i de Recerca, Grant Number: 2014FI_B00415; Comissió
Funding Information:
Interdepartamental de Recerca i Innovació Tecnològica, Grant Number: 2009SGR-00326, CMRB Promt-0901; ADVANCE (CAT) (ACCIÓ (Catalonia Trade & Investment; Generalitat de Catalunya)); European Community under the Catalonian ERDF operational program 2014-2020; MINECO grant MOLIMAGLIO, Grant Number: SAF2014-52332-R; FI-DGR (Generalitat de Catalunya) (to F.F.)
Funding Information:
The authors wish to thank CBATEG for the donation of dogs and Ester Blasco Ortega, Lola Pérez Rodríguez, Tamara Rivero Balsera, and Patricia García Tárraga for technical assistance. This study was supported by grants from the Ministerio de Economia y Competitivi-dad (PLE2009-0089, SAF2012–37417), Spain (SAF2015–66505-R to J.M.C.), Instituto de Salud Carlos III-Subdirección General de Eval-uación (RD06/0010/0006), and European Regional Development Fund (ERDF) [CIBERNED, to J. A and C.V.-A.; and RETICS (RD12/ 0019/0002; Red de Terapia Celular), to J.M.C.], Generalitat de Cata-lunya (2014SGR-968), and La Marató TV3 (20140130/1), Agència
Funding Information:
Comissió Interdepartamental de Recerca i Innovació Tecnològica, (2009SGR-00326, CMRB Promt-0901). This work has been developed in the context of ADVANCE(CAT) with the support of ACCIÓ (Catalonia Trade & Investment; Generalitat de Catalunya) and the European Community under the Catalonian ERDF operational program 2014–2020. Funding also came from MINECO grant MOLIMA-GLIO (SAF2014–52332-R). F.F. held an FI-DGR grant from the Generalitat de Catalunya.
Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - The mammalian ventricular-subventricular zone (V-SVZ) presents the highest neurogenic potential in the brain of the adult individual. In rodents, it is mainly composed of chains of neuroblasts. In humans, it is organized in layers where neuroblasts do not form chains. The aim of this study is to describe the cytoarchitecture of canine V-SVZ (cV-SVZ), to assess its neurogenic potential, and to compare our results with those previously described in other species. We have studied by histology, immunohistochemistry (IHC), electron microscopy and neurosphere assay the morphology, cytoarchitecture and neurogenic potential of cV-SVZ. Age groups of animals were performed. Histological and ultrastructural studies indicated that the cV-SVZ is organized in layers as in humans, but including migratory chains as in rodents. Neural progenitors were organized in niches in the subependymal area and a decline in their number was observed with age. Adult-young dogs contained migratory cells capable to expand and differentiate in vitro according with previous results obtained in rodents, primates, humans, pigs, and dogs. Some adult animals presented perivascular niches outside the V-SVZ. Our observations evidence a great similarity between canine and human V-SVZ indicating that the dog may be better representative of neurogenic events in humans, compared with rodents. Accordingly with our results, we conclude that dogs are a valuable animal model of adult neurogenesis in comparative and preclinical studies.
AB - The mammalian ventricular-subventricular zone (V-SVZ) presents the highest neurogenic potential in the brain of the adult individual. In rodents, it is mainly composed of chains of neuroblasts. In humans, it is organized in layers where neuroblasts do not form chains. The aim of this study is to describe the cytoarchitecture of canine V-SVZ (cV-SVZ), to assess its neurogenic potential, and to compare our results with those previously described in other species. We have studied by histology, immunohistochemistry (IHC), electron microscopy and neurosphere assay the morphology, cytoarchitecture and neurogenic potential of cV-SVZ. Age groups of animals were performed. Histological and ultrastructural studies indicated that the cV-SVZ is organized in layers as in humans, but including migratory chains as in rodents. Neural progenitors were organized in niches in the subependymal area and a decline in their number was observed with age. Adult-young dogs contained migratory cells capable to expand and differentiate in vitro according with previous results obtained in rodents, primates, humans, pigs, and dogs. Some adult animals presented perivascular niches outside the V-SVZ. Our observations evidence a great similarity between canine and human V-SVZ indicating that the dog may be better representative of neurogenic events in humans, compared with rodents. Accordingly with our results, we conclude that dogs are a valuable animal model of adult neurogenesis in comparative and preclinical studies.
KW - RRID:AB_10013382
KW - RRID:AB_10141047
KW - RRID:AB_2142367
KW - RRID:AB_2210524
KW - RRID:AB_2313665
KW - adult neurogenesis
KW - cell culture
KW - cytoarchitecture
KW - dog
KW - histology
KW - ventricular-subventricular zone
UR - http://www.scopus.com/inward/record.url?scp=85040346627&partnerID=8YFLogxK
U2 - 10.1002/cne.24365
DO - 10.1002/cne.24365
M3 - Article
C2 - 29205371
SN - 0021-9967
VL - 526
SP - 721
EP - 741
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 4
ER -