TY - JOUR
T1 - Characterization of nonochratoxigenic strains of Aspergillus carbonarius from grapes
AU - Cabañes, F. J.
AU - Bragulat, M. R.
AU - Castellá, G.
N1 - Funding Information:
We thank C. Gómez for her valuable technical assistance and P. Battilani, A. D. Hocking, A. Lebrihi, A. Lichter, E. Tjamos and A. Venancio for kindly providing us with some of the strains used in this study. This research was supported by the Ministerio de Ciencia e Innovación of the Spanish Government ( AGL2010-22182-C04-02 )
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/12/1
Y1 - 2013/12/1
N2 - Aspergillus carbonarius is the main responsible source of ochratoxin A (OTA) in food commodities such as wine, grapes or dried vine fruits from main viticultural regions worldwide. Besides, OTA production is a very consistent property of this species and for this reason atoxigenic isolates of A.carbonarius are very rarely found in natural environments. In the present study, for the first time, three nonochratoxigenic wild strains of A.carbonarius have been discovered, unambiguously identified, characterized in deep and compared to ochratoxigenic strains of the same species. In addition, polyketide synthase (pks) genes suggested to be involved in OTA biosynthesis were also screened in these strains. The identification of the strains was confirmed by ITS-5.8S rRNA, β-tubulin and calmodulin gene sequencing. The three atoxigenic strains did not produce OTA in a conducive culture medium at any of the temperatures and times of incubation tested. Five ketosynthase domains from pks genes previously described in A.carbonarius were detected both in ochratoxigenic and in nonochratoxigenic strains. Atoxigenic strains of A. carbonarius could be useful as biotechnological agents to be used in food industry and as biological agents for control of OTA production in vineyards and other crops.
AB - Aspergillus carbonarius is the main responsible source of ochratoxin A (OTA) in food commodities such as wine, grapes or dried vine fruits from main viticultural regions worldwide. Besides, OTA production is a very consistent property of this species and for this reason atoxigenic isolates of A.carbonarius are very rarely found in natural environments. In the present study, for the first time, three nonochratoxigenic wild strains of A.carbonarius have been discovered, unambiguously identified, characterized in deep and compared to ochratoxigenic strains of the same species. In addition, polyketide synthase (pks) genes suggested to be involved in OTA biosynthesis were also screened in these strains. The identification of the strains was confirmed by ITS-5.8S rRNA, β-tubulin and calmodulin gene sequencing. The three atoxigenic strains did not produce OTA in a conducive culture medium at any of the temperatures and times of incubation tested. Five ketosynthase domains from pks genes previously described in A.carbonarius were detected both in ochratoxigenic and in nonochratoxigenic strains. Atoxigenic strains of A. carbonarius could be useful as biotechnological agents to be used in food industry and as biological agents for control of OTA production in vineyards and other crops.
KW - Aspergillus carbonarius
KW - Atoxigenic strains
KW - Biological control
KW - Biotechnological agents
KW - Ochratoxin A
KW - Wine
UR - http://www.scopus.com/inward/record.url?scp=84878900871&partnerID=8YFLogxK
U2 - 10.1016/j.fm.2013.05.004
DO - 10.1016/j.fm.2013.05.004
M3 - Article
C2 - 24010591
SN - 0740-0020
VL - 36
SP - 135
EP - 141
JO - Food Microbiology
JF - Food Microbiology
IS - 2
ER -