Characterization of Necrotizing Lymphadenitis Associated with Porcine Circovirus Type 2 Infection

I. Galindo-Cardiel, L. Grau-Roma, M. Pérez-Maíllo, J. Segalés

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Necrotizing lymphadenitis is observed in approximately 2% of pigs affected by post-weaning multisystemic wasting syndrome (PMWS). The pathogenesis of the lesion has been linked to apoptosis induced by porcine circovirus type 2 (PCV2). The aim of the present study was to gain further insights into PCV2-associated lymphoid necrosis in pigs with PMWS. Three groups of animals were studied: (1) PMWS-affected pigs with necrotizing lymphadenitis (n = 5), (2) PMWS-affected pigs without necrotizing lymphadenitis (n = 5) and (3) healthy pigs with no PMWS-related lesions (n = 5). Investigations performed included immunohistochemical evaluation of the expression of cleaved caspase-3 and von Willebrand factor, Mallory's staining for fibrin and in-situ hybridization for detection of the PCV2 genome. The results of the study suggested that lymphoid necrosis in PMWS-affected pigs may be related to hypertrophy and hyperplasia of high endothelial venules (HEVs). The mechanism underlying these changes in HEVs was not clearly defined, but necrotizing lymphadenitis in pigs with PMWS may develop following vascular damage with thrombosis and subsequent follicular necrosis. Apoptosis was not found to be involved in lymphocyte depletion in PMWS or in PMWS-associated necrotizing lymphadenitis. © 2010 Elsevier Ltd.
Original languageEnglish
Pages (from-to)63-69
JournalJournal of Comparative Pathology
Publication statusPublished - 1 Jan 2011


  • Apoptosis
  • Necrotizing lymphadenitis
  • Porcine circovirus type 2
  • Post-weaning multisystemic wasting syndrome
  • Von Willebrand factor


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