Abstract
Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma IMS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed. © 1994 Institut pasteur/elsevier paris.
Original language | English |
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Pages (from-to) | 533-539 |
Journal | Research in Immunology |
Volume | 145 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1 Jan 1994 |
Keywords
- Acrosome
- Anticorps monoclonal
- Gamete recognition.
- Homme
- Human
- Monoclonal antibody
- Mots-clés Sperme
- Reconnaissance des gamètes.
- Specificity
- Sperm
- Spécificité