Characterization of monoclonal antibodies specific for human sperm : effect of CRL-10 on acrosome reaction

V. García-Framis; E. Martín-Lunas; A. Iborra; P. Andolz; M. A. Bielsa; P. Martinez

doi:10.1016/S0923-2494(94)80071-5

Characterization of monoclonal antibodies specific for human sperm : effect of CRL-10 on acrosome reaction

V. García-Framis, E. Martín-Lunas, A. Iborra, P. Andolz, M. A. Bielsa, P. Martinez

Institute of Biotechnology and Biomedicine "Vicent Villar Palasi" (IBB)

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma IMS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed. © 1994 Institut pasteur/elsevier paris.

Original language	English
Pages (from-to)	533-539
Journal	Research in Immunology
Volume	145
Issue number	7
DOIs	https://doi.org/10.1016/S0923-2494(94)80071-5
Publication status	Published - 1 Jan 1994

Keywords

Acrosome
Anticorps monoclonal
Gamete recognition.
Homme
Human
Monoclonal antibody
Mots-clés Sperme
Reconnaissance des gamètes.
Specificity
Sperm
Spécificité

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title = "Characterization of monoclonal antibodies specific for human sperm : effect of CRL-10 on acrosome reaction",

abstract = "Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma IMS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed. {\textcopyright} 1994 Institut pasteur/elsevier paris.",

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author = "V. Garc{\'i}a-Framis and E. Mart{\'i}n-Lunas and A. Iborra and P. Andolz and Bielsa, {M. A.} and P. Martinez",

year = "1994",

month = jan,

day = "1",

doi = "10.1016/S0923-2494(94)80071-5",

language = "English",

volume = "145",

pages = "533--539",

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TY - JOUR

T1 - Characterization of monoclonal antibodies specific for human sperm : effect of CRL-10 on acrosome reaction

AU - García-Framis, V.

AU - Martín-Lunas, E.

AU - Iborra, A.

AU - Andolz, P.

AU - Bielsa, M. A.

AU - Martinez, P.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma IMS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed. © 1994 Institut pasteur/elsevier paris.

AB - Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma IMS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed. © 1994 Institut pasteur/elsevier paris.

KW - Acrosome

KW - Anticorps monoclonal

KW - Gamete recognition.

KW - Homme

KW - Human

KW - Monoclonal antibody

KW - Mots-clés Sperme

KW - Reconnaissance des gamètes.

KW - Specificity

KW - Sperm

KW - Spécificité

U2 - 10.1016/S0923-2494(94)80071-5

DO - 10.1016/S0923-2494(94)80071-5

M3 - Article

VL - 145

SP - 533

EP - 539

IS - 7

ER -

Characterization of monoclonal antibodies specific for human sperm : effect of CRL-10 on acrosome reaction

Abstract

Keywords

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