Characterization of monoclonal antibodies specific for human sperm : effect of CRL-10 on acrosome reaction

V. García-Framis, E. Martín-Lunas, A. Iborra, P. Andolz, M. A. Bielsa, P. Martinez

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3 Citations (Scopus)

Abstract

Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma IMS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed. © 1994 Institut pasteur/elsevier paris.
Original languageEnglish
Pages (from-to)533-539
JournalResearch in Immunology
Volume145
Issue number7
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • Acrosome
  • Anticorps monoclonal
  • Gamete recognition.
  • Homme
  • Human
  • Monoclonal antibody
  • Mots-clés Sperme
  • Reconnaissance des gamètes.
  • Specificity
  • Sperm
  • Spécificité

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