Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France)

Aude Fourçans, Tirso García De Oteyza, Andrea Wieland, Antoni Solé, Elia Diestra, Judith Van Bleijswijk, Joan O. Grimalt, Michael Kühl, Isabel Esteve, Gerard Muyzer, Pierre Caumette, Robert Duran

Research output: Contribution to journalArticleResearchpeer-review

98 Citations (Scopus)

Abstract

A photosynthetic microbial mat was investigated in a large pond of a Mediterranean saltern (Salins-de-Giraud, Camargue, France) having water salinity from 70‰ to 150‰ (w/v). Analysis of characteristic biomarkers (e.g., major microbial fatty acids, hydrocarbons, alcohols and alkenones) revealed that cyanobacteria were the major component of the pond, in addition to diatoms and other algae. Functional bacterial groups involved in the sulfur cycle could be correlated to these biomarkers, i.e. sulfate-reducing, sulfur-oxidizing and anoxygenic phototrophic bacteria. In the first 0.5 mm of the mat, a high rate of photosynthesis showed the activity of oxygenic phototrophs in the surface layer. Ten different cyanobacterial populations were detected with confocal laser scanning microscopy: six filamentous species, with Microcoleus chthonoplastes and Halomicronema excentricum as dominant (73% of total counts); and four unicellular types affiliated to Microcystis, Chroococcus, Gloeocapsa, and Synechocystis (27% of total counts). Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments confirmed the presence of Microcoleus, Oscillatoria, and Leptolyngbya strains (Halomicronema was not detected here) and revealed additional presence of Phormidium, Pleurocapsa and Calotrix types. Spectral scalar irradiance measurements did not reveal a particular zonation of cyanobacteria, purple or green bacteria in the first millimeter of the mat. Terminal-restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA gene fragments of bacteria depicted the community composition and a fine-scale depth-distribution of at least five different populations of anoxygenic phototrophs and at least three types of sulfate-reducing bacteria along the microgradients of oxygen and light inside the microbial mat. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)55-70
JournalFEMS Microbiology Ecology
Volume51
Issue number1
DOIs
Publication statusPublished - 27 Dec 2004

Keywords

  • Bacterial community composition
  • Bacterial diversity
  • Biomarkers
  • Confocal microscopy
  • Microbial mat
  • Microsensors

Fingerprint Dive into the research topics of 'Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France)'. Together they form a unique fingerprint.

Cite this