Characterization of an oil-degrading Microcoleus consortium by means of confocal scanning microscopy, scanning electron microscopy and transmission electron microscopy

Elia Diestra, Antonio Solé, Mercedes Martí, Tirso Garcia De Oteyza, Joan O. Grimalt, Isabel Esteve

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16 Citations (Scopus)

Abstract

A consortium of microorganisms with the capacity to degrade crude oil has been characterized by means of confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The analysis using CLSM shows that Microcoleus chthonoplastes is the dominant organism in the consortium. This cyanobacterium forms long filaments that group together in bundles inside a mucopolysaccharide sheath. Scanning electron microscopy and transmission electron microscopy have allowed us to demonstrate that this cyanobacterium forms a consortium primarily with three morphotypes of the heterotrophic microorganisms found in the Microcoleus chthonoplastes sheath. The optimal growth of Microcoleus consortium was obtained in presence of light and crude oil, and under anaerobic conditions. When grown in agar plate, only one type of colony (green and filamentous) was observed. © FAMS, Inc.
Original languageEnglish
Pages (from-to)176-180
JournalScanning
Volume27
Issue number4
Publication statusPublished - 1 Jul 2005

Keywords

  • Confocal laser scanning microscopy
  • Crude oil
  • Microcoleus consortium
  • Scanning electron microscopy
  • Transmission electron microscopy

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